...depending on the model of water bath, but will likely involve a dial or a digital display you can set to the ...

...centrifuge. Immediately stop the centrifuge and investigate the issue. When you are finished spinning your...

...propagation. The majority of ligation reactions involve DNA fragments that have been generated by restriction...

...gene. This trick can also enable replacement of "inverse PCR" reactions with a two-part Gibson reaction ...

... 10 μg/mL polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each...

... 10 µg/mL polybrene. Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension (i.e...

...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Hum Gene Ther Methods...

...Addgene, we consider runs with an NTC > 10 to be invalid. To reduce NTC values, we recommend wiping down...

...Magnesium Chloride Close the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize...

...pre-chilled cuvette. Pulse. *Caution* Electroporation involves the use of high voltages, please use caution when...

...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue...

...clues about the types of neurons involved or whether their involvement is activating, inhibiting, or modulating... another. In addition to the neuronal networks involved in directly controlling muscle activity and coordinating... reflect motivational and other aspects may be involved. For example, an animal moving around to explore...norepinephrine to promote vigilance. A recent study investigated the injection of AAV8 particles expressing an...addiction and craving (‘seeking drugs’).  What’s involved in locomotor sensitization? Previous research ...circuit, and the medial prefrontal cortex (mPFC) are involved. Neurons from the mPFC project to the NAc, where....   A study from 2014 (Howerton at al. 2014) investigated the connection between neuroinflammation and...

...reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of the DNA ...recombinase, which is named after its ability to invert or ‘flip DNA’, is derived from the yeast S. cerevisiae...Recombination between these sites can lead to excision, inversion, and translocation of DNA in a similar fashion... Mutated FRT and loxP sites in Flex Vectors – inversion only once All recombination events mediated by...target sites have been engineered to avoid this re-inversion issue. It became possible to design FLEx vectors...vectors (Flip-excision vectors) that only allow one inversion event when it was discovered that it is not the... FLEx vectors, the DNA sequence that should be inverted is flanked by two pairs of target sites that differ...

...following strand invasion and D loop formation, the newly synthesized portion of the invasive strand is displaced...processed end of the non-invading strand at the other DSB end. The 3’ end of the non-invasive strand is elongated... one invading strand at a DSB that can be used for repair (a one-ended DSB). This single invasive strand...the canonical homology-independent pathway as it involves the direct ligation of sticky or blunt ends and...both a substrate for proteins required for strand invasion and a primer for DNA repair synthesis. The ssDNA...DSBR) In the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve as a primer...branched structures that form when elongation of the invasive strand “captures” and synthesizes DNA from the...

...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for...Encoded Calcium Sensors). We are expanding the inventory of these vectors to more serotypes. We currently... GCaMP7f and GCaMP7s. See all our biosensors inventory, and check out our latest vectors below: pGP-...growing field that we aim to support by growing our inventory of recombinases and recombinase-dependent vectors...

...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype...always excited to add new control AAVs to our inventory since we know that scientists need these tools...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...Dana et al., 2018). See our full biosensor AAV inventory New glutamate sensors Plasmid Serotype Name.../ Chemogenetics AAV We have also expanded our inventory of chemogenetic AAV for activating and inhibiting...be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs ...

...gained invaluable experience and even after 5 years, was still improving my skills. My involvement with ...leadership training began with the NPA where I gained invaluable experience working on and later chairing the ...already have some leadership experience). I was involved with postdoctoral issues first at Brigham and ...many people at that conference, I later became involved with the NPA beyond the meeting. I first joined...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within... the Committee and after a year of my active involvement, I am now Chair-Elect of that Committee. Being...

...enzymes and these different Dicer-like enzymes are involved in the biogenesis of different small RNAs (Figure...Typically, the desired target of RNAi is expressed as inverted repeats separated by a loop region that facilitates..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing...present at the first stage of an RNAi-based investigation. Creation of the RNAi trigger requires a few...hairpin RNA The easiest approach to induce RNAi involves the expression of long hairpin RNA (hpRNA). This...of ~400 bp complementary to your gene target as inverted repeats (Figure 1). With this technique, multiple...efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved, the cleaved...

...of the pathways involved in the development of cancer. With hundreds of genes involved in the Ras pathway... is circumvented because of the specific genes involved. Downregulation of tumor suppressors and upregulation...on picking apart the complex cellular pathways involved in the development of different cancers and identifying...identifying the roles of key proteins involved, with the goal of being able to discriminately and effectively...pathway alone and thousands of investigators studying them, how do we ensure that the research community...Cancer Research Plasmids and Resources page, investigators may select a pathway and identify any genes ... click on a gene of interest will direct the investigator to a list of all available Addgene plasmids ...

...give you an idea of what a career in this field involves, and why you might enjoy doing it. I’ll also talk...to tackle, depending on the part of the company involved: Research & Development (R&D) teams at pharma... such teams to help make decisions about R&D investments in either new internal efforts, or through acquisitions...kinds of projects. Almost all of my projects, have involved enriching conversations with R&D, Medical Affairs...examples, and healthcare consulting firms can be involved in many similar activities depending on the expertise... Such a decision determines whether the client invests substantial monetary and human resources towards...like that would allow the client to prioritize investments over the next 3, 5, or 10 years. Complicating...