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Addgene
Showing: 41 - 60 of 142 results
  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...volume and concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks...
  2. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... despite the evolving export/import rules. Our shipping and lab teams continue to keep up with the demand...have confidence that from Business Development to Shipping we will execute any new projects with our usual...
  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...
  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ... Growing the transformed agrobacterium culture Dipping your plant’s flowers in the agrobacterium culture...resuspend them in transformation media. If you are dipping your plants, put the culture into a container that...
  5. DNA Purification Without a Kit

    Type
    Blog Post
    ...and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic acids. ..., purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer, where...
  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...
  7. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...performed the following modifications to pDS132 (Philippe et al., 2004): Replaced the sacB cassette with...PMID: 29760772. PubMed Central PMCID: PMC5946454. Philippe, Nadège, et al. "Improvement of pCVD442, a suicide...
  8. CRISPR Guide

    Type
    Guide
    ...case of DNA libraries, the CRISPR library will be shipped at a concentration that is too low to be used in...from promoters and enhancers. 2015. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura...
  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9>AAV5>AAV8>AAV1>AAV6>AAV2 Auditory Cortex...upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain (4). An example...
  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...
  11. Antibody Guide

    Type
    Guide
    ...price, and other factors like availability and shipping time. Choose antibodies from companies that are...several to compare, or select one based on price, shipping availability, ease of purchase, or similar factors...
  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...gRNA/Cas9 to target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab. ...
  13. Cre-lox system

    Type
    Collection
    ...T2A-sfGFP-iCre-ERT2 sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable linker) - Tamoxifen inducible CAG Mammalian Capecchi...
Showing: 41 - 60 of 142 results