...AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells...

...HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Genetics. 2014 Dec;198(4):1347-56...

... (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara, et al., Programmable...

...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn...

...References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided ...

...Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna...

..., B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez...

...6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998...

...is then tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets...

... Lee C-YS, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome...

...cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...

...dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with puromycin...

...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; see panel A to the right). Additionally, more elaborate...Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna...

...Addgene. Hochbaum, et al. Nat Methods. 2014. Kralj, et al. Nat Methods. 2011. High-throughput luciferase...

...Plasmid Description Tet on or off PI 11643 pLVCT-tTR-KRAB 2nd generation; Transgene (CAG promoter) ‐ OR ‐ ...Patrick Aebischer and Didier Trono 11779 pLVCT-rtTR-KRAB-2SM2 2nd generation; Transgene (CAG promoter) ‐ ...Patrick Aebischer and Didier Trono 11644 pLVET-tTR-KRAB 2nd generation; Transgene (hEF-1alpha promoter) ...Patrick Aebischer and Didier Trono 11642 pLVPT-tTR-KRAB 2nd generation; Transgene (hPGK promoter) ‐ OR ‐...Patrick Aebischer and Didier Trono 11652 pLVPT-rtTR-KRAB-2SM2 2nd generation; Transgene (hPGK promoter) ‐...Patrick Aebischer and Didier Trono 11651 pLVUT-tTR-KRAB 2nd generation; Transgene (hUbiquitin promoter) ...Patrick Aebischer and Didier Trono 11648 pLVPRT-tTR-KRAB 2nd generation; Transgene (hPrion promoter) ‐ OR...

... addressed to [email protected] or vladimir.nekrasov@tsl.ac.uk . These plasmids are described in...benthamiana using Cas9 RNA-guided endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S....system. Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V. Plant Methods . 2013 Oct 11;9(1):39. PubMed...

...cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence...construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) ...

...SIP-145, hp51CN JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...D12S2489E, FLJ17759, FLJ75772, KLR, NKG2-D, NKG2D KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...MGC126258, PSCTK2 JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LILRB3 leukocyte immunoglobulin-... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells... factor X-associated ankyrin-containing protein ANKRA1, BLS, F14150_1, MGC138628, RFX-B RFXAP regulatory...

...fused to 2x NLS and tagBFP 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter...promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain 46912 pMSCV-LTR-dCas9-VP64-BFP Human expression...

...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; see panel A to the right). Additionally, more elaborate...Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna...