...sgRNAs targeting KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template...targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains a Kras G12D homology directed...ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL...Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly mutated...Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to macroscopic tumors of...

...TCTGAAAAAGACTCTGCAtgggaatggcctgcctTACGATGACAGAAATGGA KRAS TAL2272 & TAL2273 TGAAAATGACTGAATATAaacttgtggtagttggaGCTGGTGGCGTAGGCAA...

...RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma viral oncogene homolog...

...GATCCACAAGTTACAATTGG 46170 cut S. pyogenes 23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article...

...Plasmid Description Tet on or off PI 11643 pLVCT-tTR-KRAB 2nd generation; Transgene (CAG promoter) ‐ OR ‐ ...Patrick Aebischer and Didier Trono 11779 pLVCT-rtTR-KRAB-2SM2 2nd generation; Transgene (CAG promoter) ‐ ...Patrick Aebischer and Didier Trono 11644 pLVET-tTR-KRAB 2nd generation; Transgene (hEF-1alpha promoter) ...Patrick Aebischer and Didier Trono 11642 pLVPT-tTR-KRAB 2nd generation; Transgene (hPGK promoter) ‐ OR ‐...Patrick Aebischer and Didier Trono 11652 pLVPT-rtTR-KRAB-2SM2 2nd generation; Transgene (hPGK promoter) ‐...Patrick Aebischer and Didier Trono 11651 pLVUT-tTR-KRAB 2nd generation; Transgene (hUbiquitin promoter) ...Patrick Aebischer and Didier Trono 11648 pLVPRT-tTR-KRAB 2nd generation; Transgene (hPrion promoter) ‐ OR...

... addressed to [email protected] or vladimir.nekrasov@tsl.ac.uk . These plasmids are described in...benthamiana using Cas9 RNA-guided endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S....system. Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V. Plant Methods . 2013 Oct 11;9(1):39. PubMed...

...cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence...construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) ...

...SIP-145, hp51CN JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...D12S2489E, FLJ17759, FLJ75772, KLR, NKG2-D, NKG2D KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...MGC126258, PSCTK2 JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LILRB3 leukocyte immunoglobulin-... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells... factor X-associated ankyrin-containing protein ANKRA1, BLS, F14150_1, MGC138628, RFX-B RFXAP regulatory...

...fused to 2x NLS and tagBFP 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter...promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain 46912 pMSCV-LTR-dCas9-VP64-BFP Human expression...

...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; see panel A to the right). Additionally, more elaborate...Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna...

...tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene...information about cloning and additional plasmids tetR-KRAB On Aebischer & Trono 16542 pBI-MCS-EGFP Expression...

...ALS Shawn Ferguson 134611 pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027 KIF5A Halo CBA ALS Marvin Bentley...sfGFP-IRES-NGN2-puro (VK_1120) SNCA GFP TRE Parkinson's Vikram Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T...sfGFP-IRES-NGN2-puro (VK_1123) SNCA GFP TRE Parkinson's Vikram Khurana 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC...sfGFP-IRES-NGN2-puro (VK_1124) SNCA GFP TRE Parkinson's Vikram Khurana 209083 pcDNA_FRT_SNCA-Avi_HygR-2A-BirA....TO_deleted). (pEHA1448) SNCA Avi CMV Parkinson's Vikram Khurana 209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2...2A-PURO_Synapsin-BirA (pEHA1623) SNCA TRE Parkinson's Vikram Khurana 209086 piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2...2A-PURO_Synapsin-BirA (pEHA1628) SNCA Avi TRE Parkinson's Vikram Khurana 210360 TMEM106B-2Strep TMEM106B Strep CMV...

...MYC-CRISPR Library 173195 Knockout Human Dzikiewicz-Krawczyk 3rd 1-7 per element 46,354 BARBEKO sgRNA Library...

...sensor for bacteria Kralj Lab Constitutive Bacterial Electrophysiology Plasmids Joel Kralj Voltage Voltage...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...for expression in monocots and dicots - Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...