... 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression of human.... 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...):766-70. Nagy Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression... human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin...single polycistronic vector. PLoS One. 2011;6(12):e28719. Gearhart Fibroblasts Neurons Lentiviral Mouse...

... Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing and nondividing cells...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...8 kb, so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for awhile...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...“Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing other... inserted into a Cas9-containing destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...

...CRISPR/Cas9-based genome engineering from a single lentiviral vector Mammalian Lentiviral expression of... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...sort this table based on: Your particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria,...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...

...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block vectors that are...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used ...