...interested in our fluorescent protein background. Download the orange fluorescent protein background.     ...ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of our lentiviral plasmids ...not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and ...Download the orange lentivirus background.         We also have ready-to-use lentiviral preps for COVID-...pooled barcoding libraries, and more. Download the green virus background.           Maybe your viral research...organism, research technique, and more. Download the green DNA background.     Since we ❤️ DNA at Addgene,...background.     If you’re working with AAV or lentivirus in your research, you might want to use one of...

...expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization...pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP/CREM floxed...within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin Mammalian Green 8404 p236 pPr-CREM CREM ...probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 p252 pPr-CREM...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian Green 12496...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...

...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression mAzamiGreen 492 505 41 5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...

...co-immunoprecipitation to assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on... Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split...mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative...NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , endosomal escape of ..., Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns... promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly/Renilla Creating ORF-Firefly...

...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...newly synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of...three possible fluorescent proteins, chosen for minimal spectral overlap, minimal protein aggregation, ...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a...degradation in vitro and in vivo. Merged green and red channel fluorescence images of C2C12 cells stably expressing... BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green fluorescence...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...

...SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic ...relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional...thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS), allowing for .../doi.org/10.1101/2020.05.04.076067    Novel fluorescent enzyme biosensors provide direct, specific and... Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating a SL-insensitive fluorophore...conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without altering that...

...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...This can be done with lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to...division. You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral...

...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based ...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and ...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1...

... DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019. ...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent protein to the ...10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular... in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...

...mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent protein...of cell-free system, capable of producing fluorescent proteins and enzymes. Image from Moore et al., 2021...validated through monitoring the expression of fluorescent proteins and oxytetracycline enzymes. Next, this ...human mRNA binding proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode...biochemical secondary energy sources to increase protein yields from 36 μg/mL to 266 μg/mL. The reaction...libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline...

...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...Purification Purify recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity...Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...

...epitopes fused to the protein of interest are recognized and thus fluorescently labeled by scFv antibodies...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter... sites for the fluorescent proteins and an inducible promoter (Plasmid 64543or 64542) When the reporter...and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins that are bound...

...colleagues identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide interest... E., et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org... al. (2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare (Preprint...marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons,...Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted Genome Editing (...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...dimerization while maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023)....

...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a. CLAP... a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling procedure...optimized strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered...study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of...FRET to study the intramolecular dynamics of the proteins in solution and when associated with microtubules...generating other SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids ...

...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...license. A naturally occurring microtubule-severing protein, Katanin is of course named for the samurai sword...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...optogenetic module to activate or recruit your protein of interest, maybe you want to inhibit it. Restricting...development and a knockout is not feasible or when the protein of interest supports a variety of processes in ...CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest and express the other separately, then...generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing it from...

...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...second-generation CRISPR lentiviral library targets nearly all human protein-coding genes, and is unique...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...of indels and thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes...code for amino acids near the N’ terminus of the protein, in order to mitigate the ability of the cell to...for amino acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional...nucleotides, restricting gRNAs to 5 – 65% of the protein coding region will still result in many dozens ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive...0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM, Younger ST, Zhivich...

...that express one of a few different fluorescent proteins (red, green, blue). If a researcher injects a ...and neuron-specific transgene expression. Fluorescent proteins are widely used for tracing and their expression... like lentivirus, but instead is contained by a capsid which is comprised of the viral proteins VP1, VP2...Maday et al., 2014). It is currently unclear which proteins facilitate transport of neuronal tracing compounds...either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally, ...biotinylated dextran amines (Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC... For predominantly retrograde tracing, albumin protein labelled with horseradish peroxidase (HRP; Kristensson...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...