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We narrowed to 8 results for: lentiviral shrna expression vector plko 1 puro

Showing: 1 - 8 of 8 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative...Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC... with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our second ...BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -...continued to see requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2....Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest...generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... handling and expression of these vectors when used in experiments. Retroviral vectors make up a large...Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding...

  4. Lentivirus Plasmids

    Type
    Collection
    ...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid...Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid ... (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See article for more...21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro... gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the...41393 pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ... into the pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1 Recommended...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP...selection Puromycin Mammalian Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO... v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible...PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2...pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen...transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection...85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression...Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-...Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA...pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene and/or shRNA. See article ...17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV promoter TetR Eric...pCW57.1 Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection...

  8. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector..., lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral vectors can ...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and... models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host...
Showing: 1 - 8 of 8 results