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We narrowed to 7 results for: lentivirus vector plko 1

Showing: 1 - 7 of 7 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene ...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...

  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...to evaluate when working with lentivirus: these viruses are based on HIV-1, which may require additional...lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro...plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty...Plasmids Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety...Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol...conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative...

  5. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...T4 polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...(PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when working in BSL-1 and BSL-2 labs...Levels One and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral... see plasmid page yes, cut S. pyogenes BFP Kuhn pLKO.1-puro U6 sgRNA BfuAI large stuffer 52628 Mammalian...lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed...expression vectors for insertion of custom gRNA target sequences. CRISPR...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from...for decades. Wild-type lentiviruses have been engineered into lentiviral vectors that can be safely used...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...
Showing: 1 - 7 of 7 results