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Showing: 1 - 20 of 27 results
  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Updated
    Oct. 13, 2019, 10:05 p.m.
    ...fluorescent reporter (ERK KTR-mRuby2)....This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods....For testing kinase inhibitors, psMEK1 and ERK KTR-mRuby2 expressing cells were incubated with an inhibitor...alter Raf1-MEK-ERK pathway signaling by using psMEK1 and an ERK kinase translocation reporter with a mRuby2...of choice, exposed to 500 nm cyan light to activate psMEK1, and the localization of ERK KTR-mRuby2 determined...
  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Updated
    Jan. 23, 2020, 6:39 p.m.
    ...Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow Bacterial...pLSSmOrange-C1 Orange Mammalian Express a gene of interest fused to the C-terminus of LSSmOrange mRuby2...the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2...
  3. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Updated
    Jan. 7, 2020, 5:55 p.m.
    ...51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry 99130 AAVrg pAAV-mDlx-NLS-mRuby2...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...
  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Updated
    Jan. 7, 2020, 5:55 p.m.
    ...Find pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry...KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2...
  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Updated
    Nov. 18, 2019, 5:03 p.m.
    ...for Rhodamine / Cy3 will work better with shorter wavelength red fluorescent proteins like TagRFP or mRuby2...fluorescent proteins for imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2...
  6. Plasmids for Optogenetics Research

    Type
    Collection
    ...mRuby2 Adesnik 109136 pCAG-eGtACR1-mRuby2-ST Inhibitory GtACR1 Mammalian Expression mRuby2 Adesnik...pCAG-CoChR-mRuby2-ST Excitatory CoChR Mammalian Expression mRuby2 Adesnik 109132 pCAG-eiC++-mRuby2...mRuby2 Adesnik 109128 pCAG-ChroME(S273A)-mRuby2-ST Excitatory ChroME(S273A) Mammalian Expression mRuby2...-ST Excitatory C1V1 Mammalian Expression mRuby2 Adesnik 109125 pCAG-ChR2-mRuby2-ST Excitatory ChR2...mRuby2 Adesnik 109127 pCAG-ChroME(F243Y)-mRuby2-ST Excitatory ChroME(F243Y) Mammalian Expression...Mammalian Expression mRuby2 Adesnik 109134 pCAG-eNpHR3.0-mRuby2-ST Inhibitory eNpHR3.0 Mammalian...Expression mRuby2 Adesnik 109135 pCAG-eArch3.0-mRuby2-ST Inhibitory eArch3.0 Mammalian Expression...
  7. Biosensor AAV Preps

    Type
    Collection
    ...-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer, Huebener, Rose...-GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer, Huebener, Rose 68720 pAAV-hSyn1...52925 pZac2.1 gfaABC1D-cyto-GCaMP6f GfaABC1D GCaMP6f none Constitutive 5 Khakh 68719 pAAV-CAG-Flex-mRuby2...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive 1, rg* Ting 68717 pAAV-CAG-Flex-mRuby2...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f 50943 pAAV-hSyn1-mRuby2...-GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer, Huebener, Rose 100833 pAAV.Syn.Flex.GCaMP6f.WPRE.SV40...-GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer, Huebener, Rose 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato...
  8. Optogenetics AAV Preps

    Type
    Collection
    ...pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeting) mRuby2 Cre dependent...-ST Syn ChrimsonR (soma-targeting) mRuby2 Cre dependent 9 Adesnik 124603New!...t/t)-TS-mCherry EF1a C1V1 (t/t) mCherry Cre dependent 9 Deisseroth 105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2...
  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40 Oliver Griesbeck AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2...GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2...GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...
  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Kepecs pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SLmCAR (Mus musculus), ChETA-mRuby2...pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pAmRuby2-P2A-GCaMP6f (Rattus norvegicus)AAVpAAV...}on-W3SLChETA-mRuby2 (Synthetic), W3SL (Synthetic)Mammalian Expression, AAVpAAVmRuby2...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pAmRuby2-P2A-GCaMP6f (Rattus norvegicus)AAVpAAV...Bonhoeffer pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pAmRuby2-P2A-GCaMP6s (Rattus norvegicus...{hCAR}off-{ChETA-mRuby2}on-W3SLhCAR (Homo sapiens), ChETA-mRuby2 (Synthetic)Mammalian Expression, AAVpAAVMyc...pAAV-TRE-DIO-eYFP-fEYFP-f (Other)AAVpAAV Gradinaru CAG-DIO-mRuby2mRuby2..., mRuby2 Kepecs pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SLhCAR (Homo...Sabatini AAV-FLEX-tmeGFP-IRES-nls-mRuby2nls-mRuby2 (Synthetic)Mammalian...}on-W3SLChETA-mRuby2 (Synthetic), W3SL (Synthetic)Mammalian Expression, AAVpAAVmRuby2...(Synthetic)Mammalian Expression, AAVpAAVMyc, mRuby2 Kepecs pAAV-hSyn-DIO...Mammalian Expression, AAV, Cre/LoxAddgene 20298EYFP Harvey pAAV-hSyn1-mRuby2...
  11. Brain Initiative Plasmid Collection

    Type
    Collection
    ...AAV genome with tet-inducible expression of the fluorescent protein mRuby2 Gradinaru...AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2...AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter Gradinaru...protein mKate2.5 from the GFAP promoter Gradinaru 99130pAAV-mDlx-NLS-mRuby2An...AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter...protein mTurquoise2 from the hSyn1 promoter Gradinaru 99126pAAV-hSyn1-mRuby2An...Gradinaru 99126pAAV-hSyn1-mRuby2An AAV genome that expresses the fluorescent protein mRuby2...99123pAAV-CAG-mRuby2An AAV genome that ubiquitously expresses the fluorescent protein mRuby2...Gradinaru 104058CAG-DIO-mRuby2AAV expression of Cre-dependent reporter mRuby2...99114pAAV-TRE-mRuby2An AAV genome with tet-inducible expression of the fluorescent protein mRuby2...99117pAAV-TRE-DIO-mRuby2An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2...Gradinaru 99130pAAV-mDlx-NLS-mRuby2An AAV genome that expresses nuclear localized mRuby2...
  12. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow Bacterial...pLSSmOrange-C1 Orange Mammalian Express a gene of interest fused to the C-terminus of LSSmOrange mRuby2...the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2...
  13. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ..., Excitation: 559, Emission: 600mRuby2-Farnesyl-5 Data Supplement mRuby2-H2B-6Localization: Nucleus.../Histones, Excitation: 559, Emission: 600mRuby2-H2B-6 Data Supplement mRuby2-H2B-C-10Localization:...: 600mRuby2-Mito-7 Data Supplement mRuby2-MyosinIIA-C-18Localization: Cytoskeleton, Excitation: 559..., Emission: 600mRuby2-MyosinIIA-C-18 Data Supplement mRuby2-Myotilin-C-14Localization: Cytoskeleton..., Excitation: 559, Emission: 600mRuby2-Myotilin-C-14 Data Supplement mRuby2-Nup50-N-10Localization:...Supplement mRuby2-ZO1-C-14Localization: Tight Junctions, Excitation: 559, Emission: 600mRuby2-ZO1-...mRuby2-Lysosomes-20Localization: Lysosome Membrane, Excitation: 559, Emission: 600mRuby2-Lysosomes-20...mRuby-Lifeact-7Localization: Actin, Excitation: 558, Emission: 605mRuby-Lifeact-7 Data Supplement mRuby2...DsRed2-C1Localization: C1 Cloning Vector, Excitation: 558, Emission: 583DsRed2-C1 Data Supplement mRuby2...mTFP1-C1Localization: C1 Cloning Vector, Excitation: 462, Emission: 492mTFP1-C1 Data Supplement mRuby2...-Mito-7Localization: Mitochondria, Excitation: 559, Emission: 600mRuby2-Mito-7 Data Supplement mRuby2...-Rab5a-7Localization: Ras GTPase, Excitation: 559, Emission: 600mRuby2-Rab5a-7 Data Supplement mRuby2...mEGFP-N1Localization: N1 Cloning Vector, Excitation: 488, Emission: 507mEGFP-N1 Data Supplement mRuby2...-Cx43-7Localization: Gap Junctions, Excitation: 559, Emission: 600mRuby2-Cx43-7 Data Supplement mRuby2...
  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...-SpHis5 - Yeast Expression mRuby2-N1 - Mammalian Expression mRuby2-C1 - Mammalian Expression eqFP611...Expression mNectarine 558 578 26 6.9 30 min Monomer pBAD-mNectarine - Bacterial Expression mRuby2...559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...
  15. Control AAV Preps

    Type
    Collection
    ...pAAV-CAG-tdTomato CAG tdTomato Constitutive 1, 2, 5, rg*, PHPeB, PHP.S Boyden 99130 pAAV-mDlx-NLS-mRuby2...Dlx NLS-mRuby2 Constitutive 1, rg* Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP Constitutive...
  16. Retrograde AAV viral preps

    Type
    Collection
    ...Dlx NLS-mRuby2 Control Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell 112677...Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-mRuby2...
Showing: 1 - 20 of 27 results