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Addgene
Showing: 41 - 60 of 806 results
  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several ...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time...
  2. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...it? how did you get there? Many of those people I met through AWIS (Association for Women in Science); ... and embedding individual diversity to get from gamete to a visibly complex - and unique - adult. My initial...
  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...between neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...there were also people ready to discuss their VR helmets, gait tracking technologies, and brain monitors...receptors to the brain, and probing how fatty acid metabolites affect brain function. These scientists pulled...
  4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...lysosomes. Though a number of biosensors existed that met some of the criteria necessary for studying endosomes...activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell populations... recent Nature Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy...be photostable over a broad pH range. The four biometric probes successfully targeted endosomes under a...modules to repress or activate gene expression, sometimes incorporating chemically inducible approaches ...
  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...Industry interviews, however, were more structured. I met with several individual and/or small groups, usually...have a theme too, which is something not inherently captured by the STAR method. I like to think of the ...stories to tell in your interview The STAR interview method is a popular way to answer behavioral interview...situation, task, action, and results. While this method seems simple, I found it hard to remember to describe...interview. Each interview is an opportunity to learn something and then apply it to the next interview. Perhaps...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...
  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...Aims section highlights each milestone that must be met in order to deem your proposal successful. Typical..., if not all, of the limitations of current methodologies. This section sets the stage for why your funded...
  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...order for FRET to occur several constraints must be met. The donor and acceptor fluorophores must be compatible... determine whether two proteins are within 10 nanometers of each other, or measure the tension in the ...Nicolas. "FRET-based molecular tension microscopy." Methods 94 (2016): 33-42. PubMed PMID: 26210398. Additional...
  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    .... I had no idea this was happening even though I met with X on a weekly basis! The silence and maybe even... opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does...
  9. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers socially and...minds, but I think many of the students learned something. Based on the initial guidelines I received from...
  10. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...all ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering, I ...experience taught me something about myself and my future career. If it wasn’t something I liked – well, I...through developing and leading a budding nonprofit, something I had zero experience with before founding CINSO...
  11. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... collaborating with your neighboring lab, you've met interesting people at conferences and you have successfully...questions become popular. There are only so many methods and machines one team can learn so linking together...associations and specific projects where you share methods and equipment between lab groups and departments...- if you are aware of a problem or a need for something, usually someone else has realized this too. Begin... about your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...
  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...Enabling Discovery Through Genomic Tools) Program and I met scientists from 21 different labs who clearly also...access to genotypic study of diverse organisms. Methods for genetic manipulation still need to be developed..., microbes, viruses, and fungi for which these methods are presently unavailable.” As stated by one of... funded labs to rapidly disseminate protocols, methods, data, and tools that result from their work. Researchers...diverse and they inhabit all habitats - even 50 meters from Old Faithful in 60 °C soils. Dendrobatidae...using Xenopus frog cell lines to develop robust methods for frog gene editing. They edit in a cell line...species monogamous. Anolis sagrei (Doug Menke) No methods were available to create targeted gene mutations...
  13. Antibody Guide

    Type
    Collection
    ...Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays ...Antibody Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows... through the indirect detection method. In the direct detection method, the signaling molecule is conjugated...Antibody Applications - Quantification Methods Quantification methods, such as western blots or ELISAs, are...output measured. This method can be performed using the indirect detection method via a conjugated secondary...sample. Controls for quantification methods Quantification methods should be run with: a positive control...included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP) and chromatin...
Showing: 41 - 60 of 806 results