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We narrowed to 19 results for: mir sensor

Showing: 1 - 19 of 19 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent sensor for real-time measurement...interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal sensors with different ITAM/SH2... new viral preps of the dopamine sensor dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs...hottest fluorescent biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest... class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor for oxytocin...engineering improved sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting... potassium sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a ...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...2019, the first generation of norepinephrine sensors was developed, named GRABNE, which helped researchers...from Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG...GRABNE is blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR...advantages of this next generation of norepinephrine sensors is their speed. nLightG is activated eight times...selectivity for norepinephrine over dopamine. All three sensors, however, did display some reaction to high concentrations...Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...brain but have been difficult to study without biosensors to directly visualize their activity. In 2019...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ... Red calcium sensor PinkyCaMP based on mScarlet PinkyCaMP a mScarlet-based calcium sensor with exceptional...FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to monitor intracellular...+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic...Genetically-Encoded Sensors. Anal Chem. 2017 Jan 3;89(1):711-719. Amy Palmer Zinc Cyan-Yellow Zinc FRET sensors ZapCY1...fluorescent sensor. PLoS One. 2017 Nov 9;12(11):e0187481. Mathew Tantama ATP QUEEN ATP sensors for live-... FRET sensors of cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP...

  4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...in the 3’ UTR by four miRNA target sites and thereby serves as a sensor of miRNA presence and activity...field further with the creation of the largest miRNA sensor collection reported to date, containing all ...PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated gene regulation...Listen to the miRNA reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized... a subset of active miRNA’s unique to HEK293FT and HeLa cell lines. When the miRNA activity profile for...Despite several years of research, the mechanisms of miRNA regulation have yet to be fully elucidated. Ron ...all 620 sequences of probable mature human miRNAs. Weiss’s collection consists of a library of reporter...

  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT ....2021.01.003. PMID: 33714753. Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing...neurons in vivo. Because they are negative-going sensors (with fluorescent signal at baseline voltage), ... specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence transgene...AAV vectors, either alone or in combination with miR124T targeting sequences, provides a simple yet valuable...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...mutants which serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-...signaling. TRUPATH Kit , ONE-GO Biosensors Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity...for enhanced NanoLuc® protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing...Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore...Taipale 12563 PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc...particularly useful for in vivo imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns... al., 2010 and Monteys et al., 2021 . TREAT : Biosensor using Renilla luciferase and a Halo tag to assay...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...-6. doi: 10.1038/nmeth.3147. Bioluminescent pH sensor Understanding intracellular pH regulation is important...events. To overcome the issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters... biology tools by engineering light-switchable sensors developed from bacterial two-component signal transduction...transcription activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent... and 4) optimizing expression of both the light sensor and the response regulator. These changes reduced...expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or...or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene deletion, ...

  8. Fluorescent Proteins: FRET

    Type
    Collection
    ...). A Guide to Fluorescent Protein FRET Pairs . Sensors , 16 (9), 1488. doi: https://doi.org/10.3390/s16091488... mCherry2-N1 miRFP670 miRFP720 642 0.14 720 87,400 0.061 5.7 13 pmiRFP670-N1 , pmiRFP720-N1 LSSmOrange... FRET-based biosensors? Check out our Biosensors Collection to find fluorescent biosensors targeting metal...FRET-based biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors with the...Plasmid Toolkit from the Grefen Lab. FRET pairs and biosensors can also incorporate organic fluorophores via...Microscopy (Link opens in a new window) Fluorescent Biosensor Database (Link opens in a new window) designed...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category...effectively deploying them in the lab somewhat mirrors the complexity of understanding their wild counterparts...large percentage of viral vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein... most commonly requested (Figure 3). Over 400 biosensors were distributed in 2023 alone. You’ll also find...in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first developed...

  10. Optogenetics Guide

    Type
    Guide
    ... optogenetics. For information on sensors, check out our Biosensors Plasmid Collection . To find optogenetics...proteins; e.g., microbial opsins and optical switches Sensors are genetically-encoded reporters of molecular ...Light-oxygen-voltage-sensing (LOV) domains are protein sensors used by many plants, microalgae, fungi and bacteria... Neurosci. 8(9):1263-8. PMID 16116447 Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sørensen ...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Li 3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries 179217-179219 Base Editing Human, Mouse...113345 Activation Human Wright 3rd 7-14 58,570 Human miRNA CRISPR Knockout Library 112200 Knockout Human Lin...

  12. Modular Cloning Guide

    Type
    Guide
    ...into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for...for constructing highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for use). Yeast...Plants Genome Engineering, CRISPR, Plant Expression Vladimir Nekrasov 95 plasmids including CRISPR/Cas nucleases...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting...Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination target sequences...expression to your cell type of interest, while miRNA target sites can be used to prevent expression in... chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious cargo...immune response against the capsids or transgenes. Sensory neurons and liver appear especially susceptible...PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice...Chaudhari, N. (2022). Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro,9(3), ...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    .... FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova...Enhanced miRFPs are monomeric and bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric...Therefore, we recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There...set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility... miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 miRFP709 https...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Vladislav Verkhusha iRFP GAF domains from miRFP709 and miRFP670, pair with common PAS domain. Requires presence...blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors Collection Split ...near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova et al. Nat...

  16. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich New viral tool ...pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral...pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral...pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral...viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE AAV5 Biosensor GENIE Project New serotype pENN.AAV.CMVs.Pl....

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 DNMT1 GFP CMV Hereditary sensory neuropathy...Hereditary sensory neuropathy type IE Bert Vogelstein 16649 DNMT1 KO Neo DNMT1 T7 Hereditary sensory neuropathy...Hereditary sensory neuropathy type IE George Daley 36383 pSMP-DNMT1_2 DNMT1 Hereditary sensory neuropathy...Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne 122668...Nowick 174247 pAPM-miR30-EIF2AK2-ts1 EIF2AK2 SFFV VWM disease Jeremy Luban 174248 pAPM-miR30-EIF2AK2-ts2 EIF2AK2...Luban 174249 pAPM-miR30-EIF2AK2-ts3 EIF2AK2 SFFV VWM disease Jeremy Luban 174271 pAPM-miR30-MATR3-ts1 MATR3...Jeremy Luban 174272 pAPM-miR30-MATR3-ts2 MATR3 SFFV ALS Jeremy Luban 174273 pAPM-miR30-MATR3-ts3 MATR3 SFFV...

  18. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...can be used to analyze cell signaling pathways or miRNA knockdown. BRET for the best of both (fluorescent... instead. This approach has even been used in biosensors where BRET reports on the change in conformation...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson...well as Ligand-Independent Functional Changes in Sensory Neurons. The Journal of Neuroscience, 36(42), 10769...
Showing: 1 - 19 of 19 results