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We narrowed to 5 results for: mito dsred construct

Showing: 1 - 5 of 5 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...assembled into a final transgenesis construct. Plasmids constructed using Golden GATEway cloning have been...the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre...Timer protein - a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to...complexity of assembling recombination of transgenesis constructs? A new cloning system combining the advantages...simplifies the cloning process for complex DNA constructs, particular for those involving recombination...Multisite Gateway cloning to generate the final construct. Up to eight entry vectors can be used for ...methods, particularly for complex transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Howarth lab created the MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein...However, the laborious construction of repair templates and guide RNA constructs, as well as the difficult... Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed through a large-scale... that results in the creation of many E. coli constructs with greatly varied levels of expression. The...can be used to easily create protein expression constructs fused with epitopes or fluorophores, generate...platform for generating both simple and complex DNA constructs Have you ever wished you could design a gene...overexpression experiment, generate the necessary constructs, and complete screening in just 3 days? Thanks...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet...enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion...visualize and quantify the fusion dynamics of mitochondria in healthy and apoptotic cells (9). PA-FPs have...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution...

  4. Retrovirus Plasmids

    Type
    Collection
    ...pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion of dsRed by Cre recombinase... recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...Use the cpFRET kit from the Pertz laboratory to construct custom biosensors. The ScEnSor Kit from the Olsson...
Showing: 1 - 5 of 5 results