We narrowed to 40 results for: nbas
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TypeBlog Post...reporter of intracellular processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they...of this blog post, GFP refers to sfGFP-GB1. Tanenbaum et al. examined the power of SunTag for single...the motor protein kinesin across microtubules. Tanenbaum et al. then tested their hypothesis that the lower...of the GCN4 scaffold. To increase expression, Tanenbaum et al. modified the GCN4 sequence to increase ...imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments...that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system for...
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Centrifugation
TypeProtocol...centrifuge is balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous...begins to wobble or shake, this could indicate an unbalanced or improperly loaded centrifuge. Immediately ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...results: On this page you can download an annotated GenBank File or a SnapGene file for the sequences listed.... If the depositor provided a custom annotated GenBank file, this can be found on the main plasmid page...relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular feature... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused to mature fluorophores, ...Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, D., Hochman, J., & Givol, D. (1972). Localization... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...Plasmid sequences or GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post... its practicality and popularity in research (Tanenbaum et al., 2014, Konermann et al., 2015, Chavez et... . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences... genetic screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for... -
CRISPR Activation: A Practical Guide
TypeBlog Post...found that SAM (Konermann et al., 2014), Suntag (Tanenbaum et al., 2014, Gilber et al, 2014), and VPR (Chavez...198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...-100834 Chan, K. Y., Jang, M. J., Yoo, B. B., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L....Huang, Q., Ding, X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...requested through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All ...parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting... -
Typing CRISPR Systems
TypeBlog Post...j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...Arbor, MI, USA 42.2808°N 83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation...study them. You can follow her at alexandrazgreenbaum.wordpress.com or on twitter @green__algae though... -
Structural Genomics Consortium Plasmids
TypeCollection...Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine... -
CRISPR Guide
TypeCollection... PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ...Research . 25 (10), 1581–1589. PMID: 26355004 Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J... -
CRISPR History and Development for Genome Engineering
TypeCollection...Biotechnol . . PMID: 27088723 Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou ... -
Tetracycline Inducible Expression
TypeCollection...reviewed 20 June 2025. References Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems for... -
Management for Scientists: Seeking Feedback
TypeBlog Post...reading try The Discipline of Teams by Jon R. Katzenbach and Douglas K. Smith or the book on which this... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post... in gene expression and fluorescence imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell...