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We narrowed to 40 results for: nbas

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  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...reporter of intracellular processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they...of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power of SunTag for single...the motor protein kinesin across microtubules. Tanenbaum et al. then tested their hypothesis that the lower...of the GCN4 scaffold. To increase expression, Tanenbaum et al. modified the GCN4 sequence to increase ...imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments...that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system for...
  2. Centrifugation

    Type
    Protocol
    ...centrifuge is balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous...begins to wobble or shake, this could indicate an unbalanced or improperly loaded centrifuge. Immediately ...
  3. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...results: On this page you can download an annotated GenBank File or a SnapGene file for the sequences listed.... If the depositor provided a custom annotated GenBank file, this can be found on the main plasmid page...relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular feature...
  4. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...
  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...
  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...found that SAM (Konermann et al., 2014), Suntag (Tanenbaum et al., 2014, Gilber et al, 2014), and VPR (Chavez...198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014...
  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...-100834 Chan, K. Y., Jang, M. J., Yoo, B. B., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L....Huang, Q., Ding, X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru...
  8. Typing CRISPR Systems

    Type
    Blog Post
    ...j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...
  9. CRISPR Guide

    Type
    Collection
    ... PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ...Research . 25 (10), 1581–1589. PMID: 26355004 Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J...
Showing: 1 - 20 of 40 results