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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32 results for: nbas

Showing: 1 - 20 of 32 results

  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...reporter of intracellular processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they...of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power of SunTag for single...the motor protein kinesin across microtubules. Tanenbaum et al. then tested their hypothesis that the lower...of the GCN4 scaffold. To increase expression, Tanenbaum et al. modified the GCN4 sequence to increase ...imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments...that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system for...

  2. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...results: On this page you can download an annotated GenBank File or a SnapGene file for the sequences listed.... If the depositor provided a custom annotated GenBank file, this can be found on the main plasmid page...relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular feature...

  3. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

  7. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...

  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences... genetic screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...found that SAM (Konermann et al., 2014), Suntag (Tanenbaum et al., 2014, Gilber et al, 2014), and VPR (Chavez...198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...-100834 Chan, K. Y., Jang, M. J., Yoo, B. B., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L....Huang, Q., Ding, X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...requested through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All ...parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting...

  12. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...

  13. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Arbor, MI, USA 42.2808°N 83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation...study them. You can follow her at alexandrazgreenbaum.wordpress.com or on twitter @green__algae though...

  15. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... in gene expression and fluorescence imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell...
Showing: 1 - 20 of 32 results