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We narrowed to 6 results for: oct4 sox2 klf4 c myc

Showing: 1 - 6 of 6 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...expression of mouse Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance ...vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes...Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids...lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem Cells ...lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming...Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions...MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53 for single plasmid ...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation...By replacing wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) ...Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving ... diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene ... monocistronic expression of either variation of Klf4 did not lead to the observed reprogramming discrepancies...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re...2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles...Zhi Ying Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been...expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus...a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ... Tet-On inducible expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation Rudolf Jaenisch... Tet-On inducible expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation Tarjei Mikkelsen...opens in a new window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives...vector to express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 ... N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral ...expression of shRNA with puromycin selection. See Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri... with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...

  6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...22645348. PubMed Central PMCID: PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth... PubMed Central PMCID: PMC4363833. 35. Eguizabal, C., et al., Dedifferentiation, transdifferentiation,...1): p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...
Showing: 1 - 6 of 6 results