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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6 results for: puromycin/1000

Showing: 1 - 6 of 6 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...

  2. Lentiviral Prep Service

    Type
    Collection
    ...targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled...targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled...targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral Expression) system...

  4. Colony Formation Titering Assay

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator... . The following day, cells were treated with puromycin. Colonies were allowed to grow in the presence...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...12,472 SAM v1 - 3 plasmid system 1000000057 (Zeocin) 1000000074 (Puromycin) Activation Human Zhang 3rd 3 ...3 70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 -...Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid...Varies Bassik Mouse CRISPR Knockout Library 1000000121 — 1000000130 Knockout Mouse Bassik 3rd 10 Varies Berger...83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093 Activation...83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092 Inhibition...Birsoy 3rd 8 20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd...
Showing: 1 - 6 of 6 results