We narrowed to 8 results for: px459

1. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would like ...U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian...efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459) V2.0 was made available through Addgene in 2015...plasmid in the top 15. Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by...pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both...sixth most popular plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin...

2. Your Top Requested Plasmid in 2016!

   Type

   Blog Post

   ...request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested...Puromycin resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone...This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor, which had a point mutation...has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name? Not included in this...

3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ...performed by transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains a site for inserting...

4. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   ...plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR/Cas system with...

5. Genomic Deletions in Mammalian Cell Lines

   Type

   Collection

   ...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and...

6. Zhang Lab CRISPR Page

   Type

   Collection

   ...PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460...

7. CRISPR Plasmids - Empty gRNA Vectors

   Type

   Collection

   ...nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459) V2.0 62988 Mammalian BbsI yes, cut S. pyogenes ...

8. Neurodegeneration Plasmid Collection

   Type

   Collection

   ...Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP...HR_Prnp_3 PRNP U6 Dementia Walker Jackson 78623 pX459_HR_Prnp_3 PRNP U6 Dementia Walker Jackson 78624 sgRNA...