We narrowed to 256 results for: px459

1. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. px459 sgAtg5

   Plasmid

   #175023

   Purpose

   CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

   Depositor

   David Tumbarello

   Article

   Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

   Insert

   ATG5 (ATG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. px459 sgFIP200

   Plasmid

   #175024

   Purpose

   CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

   Depositor

   David Tumbarello

   Article

   Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

   Insert

   RB1CC1 (RB1CC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX459-gR2A_Hinge

   Plasmid

   #124811

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Cas9 – gRNA_R2A_hinge
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   R166H in PuroR (please see depositors comment bel…

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459 EQR

   Plasmid

   #101732

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-dCTNNA1

    Plasmid

    #176032

    Purpose

    A knock-out vector for dog CTNNA1 (alpha-1-catenin)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-sgRNA047_Podxl

    Plasmid

    #232932

    Purpose

    Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Podxl S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-dErbB4

    Plasmid

    #197357

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-dErbB1

    Plasmid

    #197354

    Purpose

    A knock-out vector for dog ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-dErbB2

    Plasmid

    #197355

    Purpose

    A knock-out vector for dog ErbB2.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459-dMET

    Plasmid

    #176031

    Purpose

    A knock-out vector for dog MET

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-dNrg1

    Plasmid

    #173696

    Purpose

    A knockout vector for the dog Nrg1.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-dEreg

    Plasmid

    #173697

    Purpose

    A knockout vector for the dog Ereg.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-dTgfa

    Plasmid

    #173698

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459-dHbegf

    Plasmid

    #173699

    Purpose

    A knockout vector for the dog Hbegf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459-dEgf

    Plasmid

    #173700

    Purpose

    A knock-out vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge