...Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How... careful not to go too far. Is the assignee interested in this task? Don’t give out tasks – where possible...

...recommend that you plate 50 μL on one plate and the rest on a second plate. This gives the best chance of...also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation... how to transform E. coli with your plasmid of interest. Protocols...

...and screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This...

... 180 regions to another or from the brain to the rest of the body. Therefore, by simply looking at how...

...the meeting and then communicate outcomes to the rest of their team. Here's a template for project meetings...

...and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

.... The two remaining plasmids in the kit have the rest of the protein in place including FokI endonuclease...new gene variants associated with conditions of interest, then studying those variants in model systems...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...

...Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a 140068 Anti-Kv1.6...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...

... translate that valuable information back to the rest of my team in Oxford (UK) to help solve those problems...realized it was a great fit for my skillset and interests. As such, I want to be able to share more about...the North America region. She is particularly interested in DNA sequencing tech, and the intersection ...

.... We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene chose this...

...target protein remains bound to the column while the rest of the proteins are eluted. Then, the antibodies...isolate antibodies only recognizing your protein of interest: affinity-purified antibodies are isolated from...

...Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed ...Zhang lab is used to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top ...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

...preparation should be doing your best to be well-rested and alert the day of the procedure. Mice move much...

...not happen in a vacuum that is isolated from the rest of society or devoid of social context. This “general...process. My role at pgEd I have always been very interested in the societal aspects of science, even while...science communication or policy requires broad interest in issues at the interface of science and society...narrowly-defined research questions in our field of interest. We often communicate in very technical and jargon-filled...jobs and those of their families. Having a broad interest and awareness of issues outside of your field ...them. Researchers currently in academia who are interested in such careers should take advantage of their...Genetics Education Project (pgEd). He is broadly interested in the legal, ethical and policy issues at the...

...locus, provided the target is unique compared to the rest of the genome and located immediately 5’ to a protospacer... What genes are important in your phenotype of interest? Many scientists study diseases for which the ...drug-resistance. Mutant cells are treated with a drug of interest and gRNA distribution is analyzed in the drug-...can help you learn a lot about your phenotype of interest. For more detailed information on CRISPR screens...

...formats helps these statements stand out from the rest of the text. Specific aims The Specific Aims section...Develop a research plan Most grant applications restrict the space available to describe the proposed work...

...release the plunger, allowing it to go back to its resting position. Wait for the liquid to stop flowing into...

...paired with dimmer dyes so they don’t “wash out” the rest of the panel and create spreading error. Conversely...

...CRISPR tools were committed to sharing them with the rest of the community. They did this by depositing them...