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We narrowed to 15 results for: spike protein

Showing: 1 - 15 of 15 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to bind...easily study the spike variants, scientists have developed ways to pseudotype the spike protein onto another...This version of the spike protein was developed based on 100 structure-guided spike designs and has improved...improved protein yields and stability. This stabilized spike protein can be used for the development of...pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids used for this study... which include a C-terminal deletion in the spike protein to improve expression and pseudotyping efficiency...(Tong et al., 2021). Another version of the spike protein, called HexoPro, was developed by Jason McLellan...

  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on...2021 with permission.   Mutations in the spike protein are one of the key drivers in determining whether... Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. ...Garcia-Beltran et al., 2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’...the current vaccines are effective or not. This protein both elicits an immune response as the target of...

  3. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient ...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's...Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant...Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus protocol for...Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped virus...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface...enter host cells. To do this, they injected the spike protein from two different coronaviruses into a llama...special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses.... sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas required. The...small (12-15 kDa) single-domain proteins that can bind effectively to protein targets, similar to how antibodies... sybodies against membrane proteins Developing antibodies against proteins embedded in membranes is tricky...candidate sybodies in their coat protein to screen for binding to the target protein. The protocol describes going...

  5. COVID-19 Resources

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    Collection
    ...-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations...MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular receptor...step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the...more. Pooled Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free...SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86...all spike expression plasmids in our collection that are suitable for generating SARS-CoV-2 spike pseudotyped...: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian...

  6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...include proteins like nonstructural proteins 1 to 16, spike proteins, envelope small proteins, and membrane...mammalian expression vectors containing various spike protein variants are also available. Plasmids are continually...length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector ...of Virus Entry Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press... Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression...membrane proteins. Although all viral components are available in bacterial expression plasmids, glycoproteins...glycoproteins and other proteins that require post-translational modification may not be easily expressed ...

  7. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike protein on the envelope plasmid. ...researchers began pseudotyping the SARS-CoV-2 spike protein into a lower BSL virus like lentivirus (Crawford...et al., 2020). That way, they can study the spike protein’s role in infecting cell lines without using ...used envelope protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric...Other envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping...Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays. Viruses 12:513 . ...different envelope glycoprotein instead of the wild-type glycoprotein. The pseudotyped protein is encoded on...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed...major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and a protein of interest... receptor binding domain (RBD) of the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library... enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target...fusion protein anchors to VP1, which self-assembles into a compartment encapsulating the protein of interest...://doi.org/10.1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast...created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus virus-like...

  10. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Browse plasmids encoding SARS-CoV-2 viral proteins....against COVID-19 all rely on DNA encoding viral proteins as a first step for many different kinds of important...enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered...coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, HKU1, and 229E) Expression...

  11. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] and Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct...the SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component ...SARS-CoV-2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M]...transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. The Britt Adamson... factors impacting prime editing and found the protein La (a small RNA-binding exonuclease protection ...prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038...immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left panel) or #211757 (middle...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-mcherry) under the control of Cre ...fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in ...In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling, researchers...be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking...is fascinated by the question of how genes and proteins coordinate billions of neurons to form the functional... Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls Sensory...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...mini counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral preps...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...our bacterial plasmid set for expressing tagged proteins, and stay tuned for mammalian expression and AAV...therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific...antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo...structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically...

  15. Optogenetics Guide

    Type
    Guide
    ...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...associate and cage a protein in the dark, while dissociate and activate the protein in the light ~400 (...
Showing: 1 - 15 of 15 results