...References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly efficient gene... (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc...19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc...

...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...

...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

... pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in ...one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description... (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs consist...

...require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ...

...synthetic biology. I've been answering biology customer service questions for nearly 7 years, and my colleagues...represented engineering first and foremost, from the custom software used to design complex genetic circuitry...

...the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are...restriction enzymes, but you will also be able to create custom annotations and design additional experiments like...

...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs.  Thanks again...

...contaminating AAV DNA. In addition, we wrote an open-source custom Python script that uses the VGS data to determine...data in minutes and the scripts  can be easily customized to detect other sequences of interest.   While...

...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic...

...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...

...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...

...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...

...listed below ( pDR274 ) can be used to construct customized gRNAs targeted to a sequence of interest. Potential...Support Tools” heading. Completed vectors express a customized guide RNA from a T7 promoter. Individual plasmids...

...listed below ( MLM3636 ) can be used to construct customized gRNAs targeted to a sequence of interest. Potential...Support Tools” heading. Completed vectors express a customized guide RNA from a U6 promoter. Individual plasmids...

... (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...