Multiplexed Overexpression of Regulatory Factors (MORF) collection
Type
Blog Post
...embryonic stem cells with the viral TF library, with each cell receiving only 1 TF expression construct. Cells...results covered TFs representing 92% of the MORF library, with an average of 206 cells per TF ORF. From this...possible TF-induced cellular states was generated (Joung et al., 2023).
Fig. 1: Barcoded TF library...performed to map TFs to cell fates. (Adapted from Joung et al.)
This atlas linked TF expression to...previously characterized TFs, the Zhang group were able to classify unknown, or ‘orphan’, TFs into their respective...number of poorly studied TFs, this functional association of the uncharacterized TFs is a useful finding.
... most immediately useful for TF library screens. Previously engineered TF libraries were not as comprehensive...