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Showing: 1 - 20 of 448 results

  1. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

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  5. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  6. TF-bio-His

    Plasmid
    #52184
    Purpose
    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TF (TF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    52184-plasmid-map-sequence-id-80548

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  13. pGreenII Tfs-qqr::LUC

    Plasmid
    #44466
    Purpose
    Expresses a luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
    Depositor
    Roger Hellens, Ross Johnson
    Insert
    A luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
    Use
    Luciferase; Plant transformation
    Tags
    Expression
    Mutation
    A disruption was made between the first and secon…
    Promoter
    35S Promoter
    Availability
    Academic Institutions and Nonprofits only
    44466-plasmid-map-sequence-id-60745

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  14. pGreenII Tfs-494::LUC

    Plasmid
    #44467
    Purpose
    Expresses a luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Depositor
    Roger Hellens, Ross Johnson
    Insert
    A luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Use
    Luciferase; Plant transformation
    Tags
    Expression
    Mutation
    A disruption was made between the first and secon…
    Promoter
    35S Promoter
    Availability
    Academic Institutions and Nonprofits only
    44467-plasmid-map-sequence-id-60747

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  19. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

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Showing: 1 - 20 of 448 results