...The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our...empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian...

...-baToN, “sender” cells express GFP on the surface and “receiver” cells express an anti-GFP nanobody on...response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern and ... nanobody cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...visualized.  The tool-kit consists of highly useful vectors compatible with Gibson and Saptrap cloning systems...for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Jacobs-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance markers...

...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...General expression mRNA Plant promoter from maize ubiquitin gene Constitutive  Gives high expression in plants...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...deacetylation. Ubc General expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  ...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...

...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... 6,000 times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the...plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR... our Viral Production Process Find other Viral Vectors ...

...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read...activation. Vary the expression levels of bait and prey proteins - overexpression can lead to false interactions...During this period, proteins were successfully expressed as fusions that retain their individual activities...see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a reporter...bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI’s interact, this...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...strains are used to select for binding-dependent expression of the HIS3 gene, which is required for histidine...

... I wanted to find a lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting ...categories Expression: Select the model system for your research (e.g. Mammalian) Vector Type: What type...Addgene is currently distributing 94 plasmids expressing validated antibodies for neuroscience research...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

...codon site of interest can be cloned into an expression vector with Gibson assembly. While this is a comprehensive...relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a ...selective pressure winnows the pool down to plasmids expressing variants with optimal function. High-throughput...ligand binding assays, phage display assays, ubiquitination activity assays, toxin activity, small molecule...is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase...2 instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...

...Fluorescent Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks...box of tags and fusion proteins to aid in the expression and purification of recombinant proteins. Tags...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding ...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Biochemists and molecular biologists who need to overexpress and purify proteins can face any number of technical... some of the hurdles to overcome in order to overexpress a recombinant protein? It is not generally in...in a cell’s best interest to overexpress a protein. Energy and cellular resources are being spent to make...

...promoter is expressed. Cre-mediated deletion of one or several of these genes permits the expression of genes...activate the expression of a different fluorescent protein. Therefore, each cell could express a unique combination... tamoxifen-inducible ubiquitin background, each cell in the mouse that expresses Cre undergoes exactly...copy of the transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with ...the low expression of XFP of Brainbow by incorporating a Tet-Off system to boost XFP expression. The increased...variety of colors when a combination of XFP are expressed. Each cell remains the same color for its entire...of the downstream OFP, GFP, or RFP. Cells that express the construct but do not undergo recombination ...

...drive gRNA expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved...Cas9 expression is commonly driven by plant-derived RNA pol II promoters which regulate expression of longer...mRNAs for gene expression). Examples of commonly used RNA pol II promoters for Cas9 expression include the...the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin promoters (Belhaj...system in plant cells. Like other model systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter...OsU6 or OsU3, which are commonly used to drive expression of small RNAs in their respective species. Addgene...with other model systems, multiple gRNAs can be expressed to modify several genomic loci at once (get more...

...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...Baculovirus expression vector with N-terminal GST pGCS-N5(GST) - GST tagged Gateway destination vector for mammalian...

...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic... lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6:2 ...

...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...CRISPR expression plasmids that have been designed for use in plants. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP...

...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...Cre expression when cloning in E. coli . This alteration enables the generation of a single vector containing...Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736...

...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity...mitophagy biosensor (retroviral expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the...

...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...associated protein C20orf188, TRRP4AP, TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, ...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...