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We narrowed to 18 results for: yellow fluorescent protein venus

Showing: 1 - 18 of 18 results

  1. Fluorescent Protein Guide: FRET

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    ...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources...for creating individual fluorescently tagged proteins to study protein-protein interactions and a series...not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...is often used to study (1) protein-protein interactions where each protein is separately fused to a donor...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion...fusion protein with your gene of interest and the listed fluorescent protein. Plasmid Color Expression Description...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...

  2. Tips for Using FRET in Your Experiments

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    ...fluorophores to proteins, pioneers of this technique were able to directly detect protein/protein interactions...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living...fused to the N-terminus of monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused ... CFP-YFP was the first to be used to study protein-protein interactions and several other pairs have been... can be used to create your choice of fluorescent fusion protein with your gene of interest: Plasmid... N1 Yellow Mammalian Express a gene of interest fused to the N-terminus of Amber Amber C1 Yellow Mammalian...

  3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    ... the Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors...other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators...Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing...expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...Cerulean or Venus Identification of new fluorescent protein fragments for bimolecular fluorescence complementation...use with mNG2(11) Improved yellow-green split fluorescent proteins for protein labeling and signal amplification...Bo Huang Yellow Venus pBiFC-VN173, pBiFC-VC155 Identification of new fluorescent protein fragments for...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to ... a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction...assemble the full structure of the fluorescent protein, producing fluorescent signal. Image modified from Feng...Multiplexed labeling of cellular proteins with split fluorescent protein tags. Tamura R, Jiang F, Xie J,...

  5. Which Fluorescent Protein Should I Use?

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    ...categories of fluorescent proteins Photoactivatable / Photoconvertible: These proteins can switch their...state of the protein is non-fluorescent, thus allowing very low background level of fluorescence. Examples... photoconvertible proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable...you, "Which fluorescent protein should I use?" And if you're looking for more fluorescence microscopy ...Interactive Visualization of Fluorescent Protein Properties  Fluorescence Spectrum Viewer  from BD bioscience...processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent proteins and their applications...Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   Fluorescent...

  6. Technologies Enabled by NanoLuc® Luciferase

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    ...biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent proteins are fantastic imaging... penetrative orange fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc...of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins. When the responsive...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by...was a fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin...Luciferase Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins...

  7. Luminescent Imaging with Nano-lanterns

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    ...Learn about Photoactivatable Fluorescent Proteins Check Out Fluorescent Protein Timers Use Rosella to Study...Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags... Since fluorescent proteins (FP) require external light activation, you can’t use fluorescence to monitor...generates much less signal than comparable fluorescent proteins. This weak emission hasn’t stopped some ...luciferase variant are used to excite the fused fluorescent protein. This technique is very similar to the widely...of low-copy number proteins, whereas such detection is difficult using fluorescent imaging due to autofluorescence... fluorescent proteins...

  8. Brain Initiative Collection

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    ...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru...

  9. Optogenetics AAV Preps

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    ...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein Activity...pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5 Bruchas 139505 pAAV-Ef1a-DIO-PPO-Venus EF1a PPO Venus Cre dependent...Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2...

  10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post
    ...If you're using green fluorescent protein or Enhanced Green Fluorescent Protein (GFP/EGFP) for a transcriptional...problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize....   1. Transmembrane fluorescent protein fusions Transmembrane proteins, i.e. receptors and transporters...However, if you fuse your protein of interest (POI) to GFP to study the protein's behavior in cells, in solution...means that your POI fused to GFP or another fluorescent protein (FP) could be forming dimers in cells. Why...interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while a protein with a high...lead to polymerization of the fusion protein. A dimeric fusion protein will have two FP binding domains forming...

  11. Luciferase Plasmid Collection

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    ...co-immunoprecipitation to assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on... Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split...mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative...NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , endosomal escape of ..., Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns...Wanker 124701 pLenti-PGK-Venus-Akaluc (neo) Akaluc hPGK Lentiviral expression of Venus-Aka-luciferase Roland...

  12. Hot Plasmids and Viral Preps - January 2021

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    ... in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the...32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput ...million cell types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP ...use light to induce conformational changes in a protein.    Cells expressing an RFP reporter plasmid...Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd) photodimers. Upon the ...High-throughput screening methods, such as fluorescence-activated cell sorting (FACS) helps researchers sift through...Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand break repair assay...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...applications of fluorescent proteins. These fusion proteins enable the observation of proteins in living cells...oligomerization of fluorescent proteins is a concern. The propensity of fluorescent proteins to oligomerize...Inserting a fluorescent protein within your protein of interest We have encountered several proteins for which... range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read our... Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse...University of Amsterdam). Tagging a protein of interest with a fluorescent protein to study its function is one...

  14. Biosensor AAV Preps

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    ...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the...Looger 106183 pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S Syn SF-Venus-iGluSnFR.A184S none Cre dependent ... Looger 106185 pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A Syn SF-Venus-iGluSnFR.S72A none Cre dependent 1... GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium...

  15. Fluorescent Protein Guide: Empty Backbones

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    ...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al....

  16. 28 Hot Plasmid Technologies from 2015

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    ...epitopes fused to the protein of interest are recognized and thus fluorescently labeled by scFv antibodies...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter... sites for the fluorescent proteins and an inducible promoter (Plasmid 64543or 64542) When the reporter...and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins that are bound...

  17. 22 Hot Plasmid Technologies from 2014

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    ...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that... synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta...three possible fluorescent proteins, chosen for minimal spectral overlap, minimal protein aggregation, ...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) ...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

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    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins are ...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of ...vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice. Current...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps, activation ...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ...why it’s used. There are two main experimental avenues (that are not mutually exclusive) commonly used...
Showing: 1 - 18 of 18 results