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Showing: 1 - 10 of 602 results
  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp...Secretion Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017, ...Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015, pp...Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, ...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, ...Acinetobacter Baumannii.” ACS Synthetic Biology, vol. 12, no. 9, American Chemical Society (ACS), 16 Aug. 2023, ...
  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs within...Note that intracellular labeling must be performed only after viability staining and surface labeling ...cell population. To detect non-surface markers, a separate intracellular labeling protocol is necessary...chemical treatment of the cells after surface labeling. Intracellular labeling can be performed on cytosolic...cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated antibodies ...on those proteins, like glycosylation patterns. Surface labeling is particularly useful for classifying...those techniques can be used alongside standard surface labels. Here, I will introduce you to a few useful...
  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional...for success prior to your departure for the new placement. Make sure you read any relevant literature for...
  4. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021...is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging phosphate oxygen. Other useful backbone...
  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...almost all of these guidelines can apply to any workplace or job. It amazes me when the most analytic scientists...thing to do is not go into these labs in the first place. This is another reason to do your research and ... Wrote blog, Toxic Academic Mentors.  What is workplace bullying? Threats to professional status – including... Scientists love learning and science so find a place that loves you back. Additional resources on the...
  6. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...orders that are placed through internal purchasing systems. All orders must be placed directly on our ... questions related to the ordering process: I placed an order through my university’s purchasing system...getting at one bigger question: How does a customer place an Addgene order using a PO number? Why do I have... organization can create an Addgene account and place an order: a purchasing agent, lab manager, or the... the contact email addresses provided. How do I place an order using a PO number? Placing an order on ...you’re using a PO number. Follow these steps to place an order online while using a PO number: Log in...
  7. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...interview with Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research... exposure to the drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers...questions that we could apply [it to].” Using ClonTracer to study drug resistance Although Bhang and co-workers...the publication, Bhang and coworkers used the ClonTracer library to determine whether or not cancer cell...the total population are easily be missed. The ClonTracer library allows a researcher to label individual...cells' genomes upon infection. When using the ClonTracer system, lentiviruses containing these barcodes...is performed via barcode sequencing. Using the ClonTracer library, Bhang et al. found that antibiotic resistant...
  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many fun things!!”  Okay - honestly.... Instead we picniced outside of our new office space on the nicely manicured lawn of University Park ... the difference here at Addgene is the emphasis placed on team building throughout the years. This team... master drywall hanger?  4. Utilize your office space too Balance point number 3 with doing fun things...
Showing: 1 - 10 of 602 results