...a group photo wearing holiday sweaters and Santa hats. There was even a giant taco... Miller lab annual...

...among the most important are the inverted terminal repeats (ITRs). ITRs are the elements that make an AAV ...guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch...2019). A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Hum Gene Ther Methods...the B-B’ and C-C’ regions of inverted terminal repeats reduces rAAV productivity but increases transgene...

... from that of wild cats with the goal of optimizing the health of domesticated cats and learning about...data for analysis. And, well, cats... everyone knows the internet loves cats! This is mostly a project to...

...Further, the pools are available in two vector formats: all-in-one (lentiCRISPRv2, expressing both Cas9...biological insight. Pooled and individual plasmid formats A first for a library at Addgene, you can also ...

...lab For more information about limitations and caveats of Cre usage: Vagaries of conditional gene targeting...

...that’s on it are plants, and the animals you eat ate plants too. Think about how many people are starving...

...endothelial cells (mice, rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No ... Mice, rats No (mechanism unknown) Krolak et al., 2022 AAV.MaCPNS1 PNS (mice and rats) CNS; ...mice) PNS (rats) CNS; PNS, including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets... not effective in NHPs (Liguore et al., 2019; Matsuzaki et al., 2018; Huang et al., 2023). However, several... mouse strains. **AAV1-X1 has not been tested in rats. For more information on reagents and protocols,... Reference AAV-PHP.B* CNS (broad) Mice, rats Yes (LY6A) Deverman et al., 2016 Hordeaux et...for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al., 2017 Brown et al, 2021...

...recommend or require authors to use specific PID formats. For example, several journals like eLife, Nature...are supported by SciCrunch. Other well known PID formats are resolvable compact identifiers, which are Compact...identifier for every plasmid. In case these PID formats are new to you, we’ll go through examples to highlight.... Despite their differences, both of these PID formats for lenti-Cas9-VQR contain the Addgene ID # and...Addgene. How to cite options using the different PID formats The instructions for how to cite any Addgene plasmid...citing with resolvable compact identifier and RRID formats. Let’s go back to our example plasmid, lenti-Cas9...happy to support the RRID and compact identifier formats. Acknowledgments Many thanks to Daniela Bourges...

..., and genetic similarities with humans. However, rats have some key differences in physiology, intelligence...edge as a disease mode (Iannaccone et al., 2009). Rats are larger than mice making them more useful for...research (Ellenbroek and Youn, 2016). For these reasons rats were once the most used mammalian model in medical... perfect. One of the most limiting attributes of rats is their difficult to manipulate genome and limited... have vastly improved scientists’ ability to use rats as disease models.  Check out Addgene’s collection...nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models & Mechanisms, 2(5–6), 206–210. https...

...parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats) on Dec 19, 2018 at 4:36am PST Buddy..., 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab materials into holiday...up about sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge...love your Insta!! #LILCChristmas . . #lab #ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #...

... Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...receptor function.     Follow them on Twitter @jakefwatson and @JGarciaNafria.  References 1. Jones, Douglas...PMCID: PMC331480. 3. García-Nafría, Javier, Jake F. Watson, and Ingo H. Greger. "IVA cloning: a single-tube...

...issue of proprietary data formats. Many instruments store raw data in formats that require specific (expensive...software that evolves over time such that prior formats are no longer supported/readable. Is the author...their host institution expected to provide data in formats exported to common file types? And how long should...

...for a ligase? DNA ligases generally prefer fully Watson-Crick base-paired dsDNA substrates to those containing... base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same...allowed screening of all possible base combinations (Watson-Crick and mismatched) around the ligation junction... (2013): 1415-1422. PubMed PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through...

...Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is ... of these arrays, with alternating spacers and repeats, was the original impetus for studying CRISPR. ...array, these sequences are called protospacers. Repeats: Bacterial DNA that appears between each spacer...

... et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos... reverse the orientation of the crRNA’s direct repeats when expressing the Cpf1 multiplex system from ...This undoes the original reversing of the direct repeats, leading to expression of a crRNA array that can...

...This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker removal so that the...second recombination event between the two flanking repeats. In cells where recombination has not occured, ...8650219. PubMed Central PMCID: PMC39188. Kamada, Katsuhiko, Fumio Hanaoka, and Stephen K. Burley. "Crystal...

...types of Class 1 TEs: those with long terminal repeats (LTRs) and those without (non-LTR TEs). LTR retrotransposons...transposase enzyme binds the transposon’s long terminal repeats (LTRs), induces a double-stranded break, and excises...generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et al., 2021).  ...

...currently at 4,651 as of April 8, 2021) Find these stats and more on the addgene.org homepage! You can track...of plasmids right below the search bar and more stats further down the page....

...season snow flurries, dusting off the winter coats and hats, and our annual Deck The Lab holiday decorating...

... community (check out the infographic below for stats on our plasmid deposits and requests). Over the ...on social media or around the office. @Addgene congrats for 1 million orders. @RothLabUNC accounts for...

...summer months, but this is also our best month ever! Hats off to all of our wonderful writers and all those...Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule...

...transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest (blue) in...is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction occurs which...

...terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an...(2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods...

...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...

... and human cell lines (Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed...conditional alleles of a few essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created...

...this promoter-gene combo are two long terminal repeats (LTRs). These LTRs define the limit of what will...constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo, in that order...

...informal settings like lab meetings, Twitter, coffee chats with potential collaborators, blogging and more....more. These each have different goals, formats, and processes, and they provide different amounts of access...

...Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright...endothelial cells across diverse mice strains and rats. Figure from Chen et al (2023) under CC-BY license...

...viral handling. Gloves are always required and lab coats are either recommended or required in BSL-1 and ...require or recommend disposable gowns over your lab coats and double gloving, so that, if needed, a layer ...

...instances I felt like I was going back to previous formats but I learnt to overcome the personal frustration...myself as a scientist. I learnt to wear different hats and came to understand the responsibilities that...

...fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka et al., 2023), but these effects were very small...5367–5372. https://doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto...

...pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking....2 (2009): 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. ...

... Can be distributed in digital or analog media formats. Cognitively demanding format. Equipment, expense...incorporate visuals if needed. Traditional media formats are quite competitive while digital can be oversaturated...

...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some of the ...doi.org/10.1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy...

...second-order structure of the AAV genome, caused by repeats and self-complementarity of the AAV genome and ...binding sites between the primers and the AAV genome repeats. Primer annealing can be affected by the presence...

...causes a shift towards antidepressant behavior in rats in the forced swim test as seen by increasing the...hippocampus evokes antidepressants-like effects in rats." Psychoneuroendocrinology 46 (2014): 78-87. PubMed...

...do identify as Democrats more often than Republicans, the March is not about Democrats or Republicans ...

...indeed the culprit in egg white injury by feeding rats purified avidin (Kresege et al., 2004). Avidin is...de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation...

..., a pooled library of scFvs is displayed on the coats of bacteriophages, a process which links genotype...pooled library of scFvs that are displayed on the coats of bacteriophages. This library is incubating with...

...large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009), and at high copy numbers...plasmid recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007...