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We narrowed to 866 results for: FER

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  1. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...premises we can classify a plasmid preparation in 3 different ways: Transformation grade DNA Cloning grade ... our Plasmids 101 blog posts Learn about the differences between transformation, transduction, conjugation...Resources on Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

  2. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    .... This is why they are called transferable skills. Why are transferable skills so important? It is important... just technical skills, but also transferable skills, before offering someone their dream job; They want...   What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...useful anywhere I work in the future; they are transferrable across most, if not all, industries and work...important to identify which transferable skills you possess because any potential employers, collaborators...certainly matters in the job market, it is the transferable skills that create a full package and add an...an extra shine to your work. Knowing your transferrable skills allows you to build on them even more, ...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...researchers learn many transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Negotiation. As a grad student,...I should negotiate my salary after receiving an offer but even as I did (successfully!), the process felt...how to spend my grant money (Can I attend this conference?) and with my lab mates on which lab jobs needed...done (Who is responsible for making the running buffer this week?). Scientists negotiate all the time ...Hawaii?). So how can you approach improving this transferable skill? Negotiation as a zero sum game Some ...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...Blog Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where...the lab can be applied anywhere! References and Resources References Grant, A. (2017). Originals how non-conformists...feel they lack formal training in a variety of transferable skills. At Addgene we're helping to fill this...Today, we’re highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists..., it’s clear that creativity is absolutely a transferable skill developed during PhD training. Creativity...inclusivity. Work with and value people who think differently from you and you will arrive at unique solutions...

  5. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...codon-optimized 613 (luciferin),730–743 (analogs) 64 Luciferin or naphthyl-luciferin analogs ATP No Deep...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...cruciata), and more. These variant proteins offer different kinetics, substrate requirements, and photon...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...or firefly luciferase into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours ...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter from a deep...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...Chiara, the tech transfer expert who understands the somewhat complicated Material Transfer Agreement process...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...such an association, start one! These teams can offer a sense of solidarity you’re going to crave. The... - with the possible exception of when Thomas Jefferson dined alone.” There was a time in human history...could know a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward... a few dozen lab meetings. The lab, as a team, suffered for it. Nobody knew what anyone else was doing...

  8. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...also matters what they’re stored in - their buffer. Buffers are made of chemicals, which have an unfortunate...the wrong everything else.      References and Resources References Boston University Flow Core. Fluorochromes... In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods...standard curve, another quick experiment, or using a different vial and saving the poor, abandoned antibody for...their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your antibodies...conjugation reaction to understand if any of your buffer components could affect the reaction. For instance...sodium azide, a common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...actually been a meeting? Different teams frequently prefer to communicate in different ways, so the source ...arbitrate for them. They were different people with some very different opinions, and some of them would...thomas-kilmann-conflict-resolution-model/    References and Resources References Thomas-Kilmann Conflict Model, Management... blog Management for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork...without my “neutral” presence to alleviate those differences, they had a hard time maintaining their friendships...the right time. Like many of the posts in our Transferable Skills series, this post is not meant to be ... that you could strengthen. The right set of transferable skills, coupled with your technical skills, ...

  10. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Problem-Solving. I don’t know about... careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined. In this ...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...your life. This tactic is a good way to see how transferable these skills really are, while also practicing...Paired with your other skills, both technical and transferable (i.e. teamwork and time management), your ability...

  11. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...resources on the Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict ...of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an...screeching feedback. You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted...remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking...most difficult form, which makes it the perfect transferable skill. Almost every other form of presenting... a clear, informative presentation, speak at conferences, or even just provide quick updates during a ...Conflict Resolution Transferable Skills Guide: Creativity...

  12. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Collaborating with others outside...collaborators from other institutions personally on conference calls and make sure to maintain these relationships... learning from others and meeting people with different mindsets and approaches can truly broaden your...

  13. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...test:  my undergrads were different people! More specifically, they had different learning styles. One was...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: managing a team. You’ve just been...motivates them (keep in mind that it can be very different from what motivates you!), know their unique skills... strengths (you will likely have reports with different levels of experience), find out what their learning...will take responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming from above...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Time Mangement for Scientists. ...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

  15. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...Before the conference Is the conference you are attending on the larger side? At large conferences, chances...once in different rooms. Before arriving at the conference, plan out your agenda. Some conferences even ... the entire conference, in general). After the conference Review your favorite conference moments on social...present your findings at a conference. But what you get out of a conference depends on what you put into...introduction. During the conference When you meet someone new at a conference, one of the first things... the conference, you can end by asking them to come to your poster or talk. After the conference After...poster session Before the conference If you’re presenting a poster at a conference, be sure to practice beforehand...

  16. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... of the conference Addgenies were lucky enough to meet hundreds of the over 11,000 conference registrants...Check Out Our Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday...involved with was one of the highlights of the conference. It was inspiring to see how microbiology research...out many of the posters being presented at the conference and talked to some scientists who stopped by ...from the nearly 4000 posters presented at the conference, it looks like there are plenty more exciting...Engineering Learn how to Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology...

  17. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...Research" conference. You can register for the conference here. The Future of Research conference is organized... insufficient (or no) emphasis on developing transferable skills to enable pursuit of these diverse opportunities...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...gradually taken over as the preferred method for many labs. However, RNA interference (RNAi) remains steadfast...umbrella term for a number of different applications that involve different types of small RNA molecules...maybe you’ll consider using RNAi! References and Resources References Bartel, D. P. (2004). MicroRNAs. ...An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still...background, a handy “oldie” as you might say. RNA interference (RNAi) is, as the name might suggest, an RNA-mediated...most popular choices for gene silencing. RNA interference RNAi is a useful tool to investigate the roles...-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA...

  19. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... attend conferences. Depending upon your discipline, there are different types of conferences that you...organizations that hold large, annual conferences. Gordon conferences offer more focused and discipline-specific...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...experiences, and several Gordon conferences are held every year in different locations all over the world...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...were so critical to me landing my own position! Conferences One of the single most important things you can...world. But simply attending the conference is usually not enough. How do you stand out from the hundreds ...

  20. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...types in the body from otherwise terminally differentiated cells simply by expressing four proteins Oct4... usually I only get to scratch the surface of different fields. This meeting has given me an opportunity...can often take the back burner at scientific conferences. In my nearly 20-year career in science, I have...

  21. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards... words, they were leaving these meetings with different interpretations of what we discussed and decided...scientists learn how to communicate their work differently based on their audience. How you talk about your...at a poster is going to vary depending on the conference you’re attending and who approaches you. You ...with professionals from other backgrounds at conferences or networking events. And if you’re a wet lab...fellow team member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell... they could in fact be interpreted in several different ways depending on the team. When Tony, our corporate...

  22. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... the conference, map out which posters you are interested in viewing. For most larger conferences, poster...ensure you have the most beneficial scientific conference experience possible. Tips for poster session ... times while standing at your poster – it’s a different experience to practice while seated or with your...Dress for the venue and professionalism of the conference, and remember to wear comfortable shoes, as you...this, and have many times answered a completely different question than the intended one. Take a deep breath...tips and tricks for presenting at scientific conferences, so let us know your own ways to successfully... this blog post for tips on other aspects of conferences Resources on the Addgene website Become of ...

  23. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...second day of the conference included workshops which were framed in advance by the conference organizing team...the amazing, postdoc-driven Future of Research Conference at Boston University last week.  This fantastic...

  24. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ... in a nonacademic direction, there are many “transferable” skills you have developed in addition to learning...your favor? Look for opportunities to practice transferable skills in ways that will also enhance your science... concrete things you can do to develop those transferable skills while you are also learning to be an ...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking... transferable skills...

  25. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases...Most AAV transfer plasmids have a promoter, but this element isn’t required when the transfer plasmid ...proteins needed for the virus to replicate,  and the transfer plasmid which contains the viral genome. In today... today’s blog post, we’ll focus on the AAV transfer plasmid and take a look at each of its parts. ... Figure 1: Parts of an AAV transfer plasmid. Parts shown in green are key elements of an AAV plasmid...structure and high GC content, but some companies now offer Sanger sequencing conditions which can read through...species or location. Promoters can also drive different levels of expression. For example, CAG is a strong...

  26. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...spending on a conference? One thing to consider is the size of the conference. Large conferences have many...make new relationships than at scientific conferences. Conferences provide the opportunity to meet people...especially when solving problems.  During the conference The conference may start in the airport. If you see ...programs. After the conference Follow up with the new people you met at the conference. If you promised ...overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups to connect...sessions that match your interests. I favor small conferences where the attendees stay onsite and eat together...face it, you see enough of them).  Before the conference Make sure you have a business card – even students...

  27. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...enzymes and these different Dicer-like enzymes are involved in the biogenesis of different small RNAs (Figure...plants, different dicer-like enzymes (DCL) process the dsRNA into specific types of interfering RNAs. ...that function downstream of DNA, such as RNA interference, can reversibly exert their effect independent...independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic process where approximately...equivalently precise, but each method recovered different genes known to be required for viability (Morgens...subsequent two. Consequently, I will focus on different approaches and considerations present at the first...been extensively used in plant research and many different constructs are available to the community. Each...

  28. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...sessions The poster session from conference to conference can be wildly different. There can be no presenter...participants used the conference hashtag after the conference was over. Depending on the conference, organizers ...scientific conferences. Plan your sessions, not your walks between sessions Like many large conferences with...can use to help plan your conference experience.One of the biggest differences: you don't have to build..., requiring you to zoom in to different parts of the poster. Conferences, such as Photonics Online Meetup..., 2020 Final thoughts on virtual conferences Virtual conferences remove many barriers of participation...spend with the conference. “If you want to have the same experience as an on-site conference, you really ...

  29. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...supporting in-person conference attendance, the award now supports virtual conference attendance. We also...Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners! ...awards help early career researchers attend a conference of their choice while honoring the late Michael...and highly interactive environment that Janelia offers,” says Aggarwal.In addition to the biosensors, ...hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the desulfuration of...an enzyme-bound hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides...redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its ...

  30. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...scientific network We refer to the process of mentoring within a bin as Horizontal Transfer. This comes from...from horizontal gene transfer, which occurs when bacteria of different evolutionary lineages share genes...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...other and what to do.  Saved by the Tweet - The conference hashtag I had, however, started a Twitter account...like anyone at a meeting who felt isolated at a conference for any reason was a Singleton. Figure ...

  31. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...is redifferentiated to a different cell type. Transdifferentiation In this process, differentiated adult...reversal of a differentiated cell type to an undifferentiated state and then redifferentiation into the cell...variety of plasmids for differentiation here. Transdifferentiation Dedifferentiation to an intermediate pluripotent...ability to convert a differentiated cell to a different cell type. While transdifferentiation is suited for ...ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is... are converted to cells of a different lineage, without dedifferentiating into a pluripotent stage. Cellular...pluripotent state. Redifferentiation - Conversion of the pluripotent cells into differentiated cells of choice...

  33. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene...largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the video...community. Not surprising, this is where most of our conference room names originated from. Let’s take a look...Groucho, a transcriptional co-repressor The first conference room, that is actually used as a break room for...diffuses down a concentration gradient exerting a different effect on the developing embryo depending on its...Surprisingly, Sleeping Beauty is not only a princess who suffered a terrible fate when she pricked her finger on...required for spermatogenesis One of our smaller conference rooms is named Cookie Monster. cookie monster...

  34. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...optimizing your transfer buffer.  Adjusting the buffer For wet and semi-dry transfers, you can adjust ...methanol concentration in transfer buffer is < 20% Wet transfer Increase transfer time Increase voltage...decrease alcohol in transfer buffer Small proteins: Over-transferring Transfer with two membranes...increase alcohol in transfer buffer Mixed proteins: Transferring issues Transfer with two membranes...be under-transferring.  Membrane pore size Membranes come in different pore sizes for different proteins...large.  Transfer method   Wet transfers are more efficient, so if you’re struggling to transfer a large...consider using a wet transfer with a long transfer time. Be aware that the transfer works by electricity...

  35. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...There are many different lysis buffers available commercially, and most common buffers have recipes available...Types of transfers Your transfer options are wet, semi-dry, or dry/electroblot. A wet transfer is the most...  Note that each type of transfer requires different equipment. Wet transfers can be done in an insert... while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the...is typically sufficient.  Lysis buffer When choosing your lysis buffer, consider the subcellular location...and Tris gels run proteins in different patterns, due to their differing chemistries. You therefore cannot... 12% 10–250 kDa Different sizes 4–12% gradient 4–250 kDa Different sizes 4–20% gradient...

  36. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...melting profiles (Dwight, 2011).  Reference Genes  Choosing a set of reference genes deserves more attention...attention than it usually receives. Different reference genes have shown to have as much as sixfold variation...Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference dye,...Liu and Saint No Raw Data Reference and target genes can have different efficacies DART-PCR No ...will soon become a qPCR master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative...mRNA, which can be used as a proxy measure for differences in protein expression. (If you're just getting...assume that qPCR can accurately detect expression differences of twofold or greater with good statistical significance...

  37. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...systems efficiently transfer a range of protein sizes in minutes without any transfer buffer. These systems...electric current. The wet transfer process is very effective in transferring a broad range of protein ...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...validated for immunoblotting. Different immunoassays detect proteins in different states. For example, in immunoprecipitation... other antibody topics. Resources and references References Aydin S (2015) A short history, principles...western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected using antibodies...

  38. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...heterogeneous population, cells behaving differently (e.g. different growth properties, migration abilities... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...targeting all differentially expressed genes that are known drug targets, or all differentially expressed ...best gRNAs by the depositing lab. The different labs use different algorithms to calculate which would ...were not found in the reference library. It’s not uncommon that genes have different names, and the software...generation sequencing to identify which gRNAs are differentially expressed in the separate groups and, as a ...is not targeted by only one gRNA, instead many different gRNAs are used for each gene. At the moment, 13...

  39. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses Plasmids 101: Optimizing...lysis buffer ratio and result in incomplete lysis. You may be able to scale the amount of buffers used ...TakaraBio) offer yeast-plasmid specific kits.  Medium/High-throughput – Many manufacturers offer bead- and...quantity.   Figure 1: Plasmid preps fall into different categories based on their relative qualities and...fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep, etc. ...what do we mean by DNA quality? There are a few different issues that can arise during the plasmid purification...which can happen if you leave the sample in a lysis buffer for too long or if you get a little overzealous...

  40. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...stable expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your...the control of two different promoters. Both the single and dual promoter transfer vectors can be used...genetically modify primary cells. Amongst the different vectors used, lentiviral vector constructs have...background Until the development of viral vectors, gene transfer into mammalian cells often relied on chemical ...These techniques are still used and efficiently transfer plasmid DNA efficiently into dividing immortalized...lines but reach their limits when it comes to transferring DNA into primary cells or non-dividing cells... particle (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert of interest as well...
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