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We narrowed to 70 results for: NIG

Showing: 1 - 50 of 70 results

  1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...sequences. Thus, the minigenome contains all cis-acting signals necessary to direct minigenome replication and...Other Important Components of EBOV Minigenome System). However, minigenomes are incapable of producing infectious...alongside a plasmid encoding the minigenome. These genes and the minigenome are transcribed (and the viral...single-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as a template...Technical Tips). Minigenome replication and transcription In order to replicate the minigenome, the viral polymerase...of the minigenome to produce the positive sense, genome replicative intermediate (anti-minigenome). The...blot analysis or RT-PCR. Uses of the minigenome system Minigenome systems, such as the one described here...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... can use for long-term library storage. After overnight growth, scrape up, pool and extract plasmid DNA...the plate so you can regrow the lifted colonies overnight), flip the membrane over and place it colony-side...tested, as with any protein purification, but overnight at 30°C is a good start. Prepare a lysis solution...repeated 5 times) works well. Leaving the membrane overnight in the freezer during one of these steps is also...with your cells of interest and growing them up overnight. Assuming they expressed well, the proteins encoded... it can really help to incubate the membranes overnight in 500 mM to 1 M NaCl in order to enhance folding...selective for your target organism and incubate overnight according to appropriate cell growth conditions...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...a new coordinator for the annual Math & Science Night, I took the reigns. I also volunteered when our ... started coordinating the annual Math & Science Night, a chance for parents and other members of the community...activities. If your school doesn’t have a Math & Science Night (or some equivalent), here are some quick tips to...activities we had for my school’s Math & Science Night. There are plenty of places to get ideas for science... Elementary for trusting me with Math & Science Night, and a special thanks to Joanne Marks, Jonathan ...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... virus infection. Microbiology and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed...biology while leaving virulent components behind. Minigenomes are stripped down viral genomes containing only... as reporter genes. To learn how you can use minigenomes in your research, check out this post! R Bodies...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... your assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people...people choose to incubate their primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs...membrane. It requires anywhere from sixty minutes to overnight to run, and is recommended for very large proteins...

  7. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...displayed!).  The Addgene themed music playing all night was hand-picked (like our plasmid samples!) by Addgenies...Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents up ...

  8. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out together. “We also ...

  9. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are perfectionists...

  12. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders...I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, ...

  14. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...Sanger sequencing is done from glycerol stocks (overnight cultures with 15% glycerol), as opposed to purified...handling of large numbers of samples. When we grow overnight cultures for glycerol stock production and storage...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...daunting. It took a true team effort and many late nights, not unlike most research, but the urgent need ... Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...respond to magnetic fields (Karki et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic... 1521–1534. https://doi.org/10.1002/pro.4124.  Kattnig, D. R., Evans, E. W., Déjean, V., Dodson, C. A....

  17. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...samples that come through our doors. It would be a nightmare if we didn’t have a simple way to track all of...bacterial glycerol stocks on Fridays. From each overnight culture, we prepare three sets of 2D barcoded ...

  18. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...to help with elder care, carpooling, cooking on nights when you can’t get home, babysitting or running...wait and some things can’t. I kiss my kids good night every evening I am home no matter how busy I am....

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID...PubMed PMID: 2536188. Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde...

  21. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...time. Though the most common conditions are 4 °C overnight or room temperature for 1–2 hours, there is some... loaded Incubate primary antibody at 4 °C overnight Incomplete washing Ensuring wash is happening...

  22. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as excited as we...

  24. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...exhibit booths, happy hour socials, and late, late night socials. As a newbie to the SfN conference, I have...

  26. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...vectors containing high numbers of gRNAs in one overnight reaction. STAgR is highly customizable as vector...

  32. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...neurons in a brain region called the substantia nigra, which is the main source of the body’s DA. Neurons...

  36. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...people pay attention. Practice Nobody gets good overnight. The only way to improve is to keep working on...

  37. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...museums, YouTube channels and podcasts, science pub nights, and pop sci books. Most scicomm efforts fall under...

  38. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...right away OR you can store the reaction at -20C overnight. 4. Transform TOPO Cloning Reaction into Competent...

  42. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... plates at 4C for few hours after the initial overnight incubation increases pigment precipitation, enhances...

  45. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, Fishman GI, Conklin BR (1999) Conditional...

  46. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...extraversion for me. Make sure to get enough sleep the night before. Have your morning coffee, if that’s part...

  47. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...required and no one lab or professor becomes an overnight success. Below I have detailed some tips to improve...

  49. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    .../10.1089/hgtb.2016.180 Korte, J., Mienert, J., Hennigs, J. K., & Körbelin, J. (2021). Inactivation of ...
Showing: 1 - 50 of 70 results