...but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing small...’d be too late to meet up, and I’d just end up spending more time in the lab. Obviously I didn’t have ...didn’t realize it until it was too late. I didn’t spend enough time thinking about the possibilities and... moderately useful tasks at the expense of time spent on the long and highly useful tasks. The quick accomplishments...complete the entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The...had general goals for my experiments, but didn’t spend enough time thinking about when or how long it would...

...budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught to communicate...the solutions they promote help motivate you to spend a little time advocating for science and getting... New Yorker, so it’s not easy to convince me to spend a Saturday in the tourist-trap nightmare that is...disconnected from the entire scientific process.  I've spent a lot of time debating how to fix this.  As scientists...W - Erin Golden Hi, my name is Erin and I have spent the last 15 years training as an academic scientist...

...arm (safe). Mice with higher IL-1 beta production spent more time in the open arm and were less risk averse... dark (protected) area. When measuring the time spent in a particular part of the apparatus, it turned...turned out that the IL-1 beta overexpressing mice spent more time in the risky bright place, driven by their...behavioral despair, and the more time the animal spends in the passive mode, the stronger is the depressive...swim test as seen by increasing the ratio of time spent actively swimming versus passive floating. In the...

... at Addgene as an Outreach Scientist. I used to spend my days (and often my nights :D) engineering E. ... Pamela Silver’s Lab (plasmid page here). Now I spend much less time wrangling bacteria and instead help...in my position as an Outreach Scientist where I spend a large amount of my time meeting with and talking...on a plane back to Boston from Kentucky where I spent a couple of days talking to researchers about their...

...A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how...determining whether an advisor will be a good mentor: Spend time with the potential advisor and don’t be shy... in the lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It...sign if the PI does not welcome you to visit and spend time with everyone. Additionally, if you are looking...

...can be a major decision. A science trainee will spend 4-7 years working for one person and with a group...gives a 1 hour talk about his or her interests, and spends one-on-one time with the current members to share...solid foundation on which to thrive during time spent as a graduate student or postdoc. Although the chosen...completely happy at work all of the time, the goal is to spend as much time in the "zone" as you can. You know ...

...experimental limitations and considerations? I recently spent two months in the lab of Antonello Bonci, Director...The technique is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables... method of identifying bregma but it is best to spend the time practicing your injections until they are...accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic frame ...

...University, you are expected to spend at least one semester (the majority spend two to three) working in your...of my six month employment. In the beginning, I spent a lot of time shadowing members of my team to learn...

...Addgene, I had spent most of my junior year stressed out applying for summer programs. I spent one day at ... before high school senior year would have been spent within a lovable community that has allowed me to...

... and reaching out if any milestones are missed. Spend time in person Presenting the results from...circumstances permit, think about having collaborators spend time with each other in person. One possibility ...possibility is to do a lab exchange, where researchers spent time in each other’s labs. This is an opportunity for...

...to make a plasmid? Or how much time you have to spend cloning before you can start your experiment? What...the most valuable component in science – the time spent by the scientists designing and performing the experiments...but we can assume it’s at least equal to the $155 spent on reagents. Five ways to decrease plasmid production...

...this method, beads are incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet...Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods Mol Biol. 2018;1850...on magnetic beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510...

...the mix. Thus, our founders and early employees spent this remaining time by elaborately pranking each...play hard.” Part of the reason we sincerely like spending time together is that when it’s time for fun, ...more manageable when you can enjoy the people you spend 40+ hours with each week. In conclusion - get out...

...future work. She graciously took me for coffee and spent an hour with me talking about the tools I could ...rapidly. The knowledge the Singletons gained from spending time with a mentor, as well as having a cohort...participants regarding the activities and time mentors spend with Singletons in their bins. We also sought expertise...

...subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings...system An electronics system  Cells are first resuspended in a pressurized buffer called sheath fluid and...instrument clogs, ensure that samples are single cell suspensions. For cells that tend to aggregate, pass the sample...

...that scientists have amazing stories to share. I spend a lot of time telling scientists about Addgene’s...how to deposit and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations...

... can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges are...

...biorender.com   Both gradients can be informative, so spend some time thinking about which type of gradient ... your blot’s data, the more time you’ll want to spend optimizing it. Blots for quantification generally...antibodies or validate them yourself, so you don’t spend time troubleshooting an assay that’s fine with antibodies...

...validated gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine...

...GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...

...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and ...Cambridge and Bristol. If you’re a scientist who spends many hours at a microscope, you too can become ...

... plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...

...scientists accomplish their goals. Many nonprofits must spend a lot of resources continuously raising money from...self-sustaining. With this unique model, we are able to spend resources on maintaining quality and starting new...

... into your interests and will determine how you spend a good chuck of your mental energy and time. The...At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

...though derivatives have been adapted for growth in suspension. Of the many derivatives of HEK-293, the most...systems, such as yeast. HEKs can also grow in suspension, which allows for large scalability, and can ...

...letters after your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking...entrepreneur at 22. Start with your network You've spent your life making connections and finding mentors...

... I was having outside of the “ivory tower.” I’d spend the afternoon lovingly tending to the stem cells...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...

...come here, I need to spend time here, this is an incredible place.” Doench has spent most of his research...

...Initially, he spent the majority of his time in the lab.  As time went on and he was spending more time in...

... makers to keep things legible. It is faster to spend more prep time on an experiment than it is to rerun...when I was tired, stressed, or rushing. I would spend the time quietly running through the experiment ...

...least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C ...products on the market to choose from) on your resuspended RNA sample will help ensure DNA contamination...

... the Scientific Support team, much of my day is spent helping to resolve problems, but even so, I wasn...framework to the myriad challenges we encounter. As you spend time in a lab learning techniques and taking on ...

... years as a senior scientist at Addgene. Having spent that long in customer service, I've daydreamed about...utility in the least amount of time. Though I now spend far less time answering help emails and phone calls...

...ultracentrifugation of the viral stock, followed by resuspension of the collected particles in a smaller volume...white pellet. The pellet can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate...

...motivation, but there very few jobs available. So, we spent a lot of time talking about what we could do to ... CSU in 2014. Outside of the lab, Keira enjoys spending time with her husband and two Jack Russell Terriers...

... wear comfortable shoes, as you will likely be spending a few hours on your feet. Pack water and snacks...you ever gone up to a poster where the presenter spends the entire time talking to the poster, as opposed...

...You’ve spent days and weeks thinking of an amazing project. You’ve written your protocols, designed your...generate templates for the cloning process. Time spent actually doing the experiments are also generally...

..., homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After finding no cross-reactivity...scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et al., 2020. ...

...plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

...and contribute to my community. This past year, I spent some time thinking about how I could combine all...science communicators.  What can CRISPR do? I spent more time on this section of the presentation because...

...agrobacterial cultures are ready, spin them down and resuspend them in transformation media. If you are dipping...transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of transformation medium per Agrobacterium...

...day, you can save the leftover bacteria-water suspension and use them to start cultures of your positive...up PCR reactions: The remaining bacteria-water suspension will serve as the template for your PCR reaction...

...think about how much of that time you’re actually spending doing work. Yes, you may legitimately need to ...interact with...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and virtually...

...circuit is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. ...

... below “Tell a story”) and note how they build suspense. Notice the simplicity of their slides and how..., you just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today...

...Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted...Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T (2017). Genome and epigenome engineering CRISPR...

...logic - even choosing a training lab where we will spend the next 6 years. Know yourself I rotated in 3... lab if at all possible. If not, see if you can spend a day or two hanging out around the lab and especially...

... your membranes with PBST to remove salt, then suspend a freshly harvested dose of cells (preferably in...enjoys pondering how phages get through the day, spending time with her horse, and wondering what her cat...

...depending on the revisions and the amount of time spent shopping a manuscript. Manuscript writing can take...fit into a future manuscript? You could easily spend a day or a week planning an experiment, even a relatively...

...committee. However, the majority of the time was spent with Addgenies meeting each other's friends and ...