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We narrowed to 77 results for: sas

Showing: 1 - 50 of 77 results

  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...of sample types (Engvall 1971). Scientists value ELISAs for their accuracy, specificity, and sensitivity...considerations when using the method. ELISA basics All ELISAs follow the same overall process. First users coat...figure was created with BioRender.com  Direct ELISAs A direct ELISA is the quickest and simplest of ...saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background staining...components and increase background staining. Direct ELISAs also lack the signal amplification afforded by ...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of...also be a source of cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...transcript. By Arunreginald at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing.../doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization ...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...References and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis...DM, Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria meningitidis (Nm ...K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically...XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015...

  9. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL....

  11. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...PowerPoint etc) or Programming skills (Python, R, SAS, HTML, Matlab etc) are highly desirable in a range...

  12. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued...

  15. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

  17. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable and reversible...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...repair using single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering...

  21. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation signal...

  22. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... McMillan L, Vridhambal GS, Rivera AJ, Montgomery SA, Fortier L-C, Barrangou R, Theriot CM, Ousterout ... lab's system called CAST (CRISPR-associated Transposase) both enable gRNA-directed transposition. INTEGRATE...DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer...

  24. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista...

  25. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...contains the transposase gene (red) for expressing the transposase enzyme.2.) The transposase enzyme is ...Beauty transposase gains its name from the fact that it was recreated from a defective transposase found...plasmid is then degraded by the cell.4.) The transposase creates a double strand break in the DNA and ...system is comprised of 2 components: 1) an SB transposase, the enzyme required for catalysis of transposition...found in fish. This transposase has been discovered in the genomes of many different fish species in a variety...synthesized by fusing and modifying various extinct transposases. Improvements have been made since then by increasing...2009, Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells...

  26. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... components, a transposon and a transposase. The piggyBac™ transposase facilitates the integration of ...controlled by titrating the transposase to transposon ratios. High ratios of transposase to transposon result... that of the wildtype piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not...plasmids, when combined with a source of piggyBac™ transposase (available from a licensed distributor) allow...cannot distribute plasmids with the piggyBac™ transposase itself, please read on to learn more about this...importantly for this technology, however, is that the transposase also enables the excision of the transposon in...virus-free. This is achieved using the piggyBac™ transposase, which facilitates direct integration of the ...

  27. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...the SB transposase, SB100X, is ~100-fold more efficient compared to first generation SB transposase (Mátés...module with the crRNA, 3) a transposase complex made up by three transposase proteins (TnsA, TnsB, TnsC...DNA site to another. This protein is either a transposase or a reverse transcriptase, depending on the ... HIV, replicate. Class I TEs do not encode a transposase enzyme.  Class I TEs are considered replicative...., 2021. To mobilize a transposon, first the transposase enzyme binds the transposon’s long terminal repeats...footprint is left behind. When the transposon-transposase complex finds its target site, it integrates,...the protein they need to move around, either a transposase or a reverse transcriptase. Non-autonomous transposons...

  28. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...their limited programmability: depending on the transposases involved, they insert either randomly, or at...combining the DNA integration capabilities of transposases and the target programmability of CRISPR-Cas...Sternberg group, and CAST (CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons...DNA-targeting module together with the crRNA.  Three transposase proteins (TnsA, TnsB, TnsC). The donor DNA (aka...RNA-DNA base pairing. Separately, TnsABC forms a transposase complex with the mini-transposon, excises it ...proteins (Fig. 1A, bottom), and require only two transposase proteins (TnsB and C). While requiring fewer ...CRISPR-transposons within the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR...

  29. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTnsABC expresses the transposases TnsA, TnsB, and TnsC pQCascade expresses the transposase TniQ, the Cas proteins...chromosomal integration using CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these...system which is based on the CRISPR-associated transposase system, INTEGRATE, is best for multicopy chromosomal...Anhydrotetracycline (aTc) induces expression transposases leading to increased integration frequency. ...Chromosomal Integration Using CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org...

  30. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...conformation changes. Several types of assays, such as ELISAs and Westerns, frequently denature the proteins ...absorbing of the proteins to the microtiter walls for ELISAs and a more accurate separation of proteins by mass...Monoclonal antibodies suitable for a Western or ELISAs may therefore often be specific to denatured versions...validated for Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western...

  31. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... quota of 85,000 total H-1B visas approved each fiscal year: 65,000 visas plus 20,000 reserved for individuals...postdoc. Foreign national researchers who require visas, permanent residence or citizenship to continue ...degree or higher degree. These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B...the life sciences community obtain their working visas, permanent resident green cards and citizenship....

  32. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control for All ...viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and...BCA assays have linear trendlines while those for ELISAs and other enzymatic assays are sigmoidal. Once ...

  33. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...called evoCAST — an evolved CRISPR-associated transposase (CAST). Back up a second. What are CASTs? CASTs...Bacterial genome engineering using CRISPR-associated transposases. Nature Protocols, 19(3), 752–790. https://doi.org... with a laboratory-evolved CRISPR-associated transposase. Science, 388(6748), eadt5199. https://doi.org...

  35. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...along with a helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest...flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, transgenes...transgenes can be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that...

  36. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer, activating... in response to the ligand, the complex will disassociate, resulting in a decreased GFP2 emission. The...

  37. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...protein (HRP), primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it...of signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that...

  38. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer. The Gα...References and resources References Kroeze WK, Sassano MF, Huang X-P, Lansu K, McCorvy JD, Giguère PM,...

  39. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

  40. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...

  41. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Types of ELISA There are four different types of ELISAs, which differ in the way the antigen is detected...available for your antigen as the other types of ELISAs require two different antibodies. As mentioned ...

  42. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. ...Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano...

  43. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better to first engineer...the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several generations...

  44. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes... optogenetic tool for localized, microtubule disassembly. Current Biology, 32(21), 4660-4674.e6. https...

  45. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    .... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA...

  46. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...in the experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

  47. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...

  48. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...
Showing: 1 - 50 of 77 results