Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 90 results for: REP

Clear keywords
Showing: 1 - 20 of 90 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...reduce binding with Rep proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important...persistence. They work in conjunction with the viral rep (replication) and cap (capsid) proteins, which bind...). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding site...resolution site that serves as the target site for Rep proteins (Savvy et al. 2013). Figure 2: AAV2 wild...-C’ regions) and forms a T-shaped structure. The Rep binding element (RBE) and terminal resolution site...productivity but increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...Created with BioRender.com   Replication (rep) gene The rep gene encodes four proteins that are required...Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins...domains, all four Rep proteins share a common helicase and ATPase domains. To function, the Rep proteins form...RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV... when recombinant AAV (rAAV) is made in the lab, rep and cap are expressed from a separate plasmid in ...virus instead.  Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid...replication and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene expression is often driven by the AAV p5 or ...

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular...site-directed mutagenesis by recombineering.” Sci Rep 6:19121. PubMed PMID: 26750263. PubMed Central PMCID...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...interconvertible Drosophila overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...entry The viral packaging proteins for AAV are rep and cap. Rep is required for viral replication and also...release from late endosomes when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015 Nov 5;5:16277. PubMed PMID: 26538064. PubMed...-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016 Mar 4;6:22555. PubMed PMID: 26939770. PubMed...-1 Replication and Accelerate Viral Escape. Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan V, Shlomai...DNA efficiently suppresses hepatitis B virus. Sci Rep. 2015 Jun 2;5:10833. PubMed PMID: 26035283. PubMed...

  9. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP...photoconversion of GFP for protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-018-33408-9 Mizuno...Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038/srep32565 Resources ...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...immunoglobulin binding domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-...
Showing: 1 - 20 of 90 results