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We narrowed to 749 results for: SOM

Showing: 1 - 20 of 749 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not thermophilic (high heat loving...editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce genomic edits in mammalian...? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...

  2. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...conjunction of vectors and exosome was given the name vexosomes (vector-exosomes), exosome AAVs, or exo-AAVs (...characterized as it is not a common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from...These AAV particles are often found with cellular exosomes, small membrane vesicles or microvesicles (approx...either associated with the outside surface of the exosome or are carried inside the vesicle, which may also...researchers discovered that these particles have some advantages over the conventionally purified AAV ...the therapeutic efficacy of AAV vectors. How are exosome AAVs produced? Purifying exo-AAVs is actually a...as CD9 is thought to enhance the production of exosomes or stabilize their structure or their interaction...

  4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...source of somatic cells for reprogramming purposes. In this post, I’ll describe an episomal vector system...virus- a non-integrating RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express...Reprogramming efficiency of PBMCs with episomal vectors Episomal vectors are based on the Epstein-Barr ...source of somatic cells for reprogramming. Enhancing PBMC reprogramming efficiency: the pCXLE episomal vector... pCXLE episomal vector system generated by the group is an optimized mixture of three episomal plasmids...expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector system increases...opportunity to develop and use patient-specific somatic cells that are otherwise difficult to obtain. These...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist...helpful, or sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages... find that there is not someone (or someones) taking on these responsibilities on a team to which you ...by offering advice on areas where you might need some help. Today: Teamwork. My first experience on a ...undergrad at Worcester Polytechnic Institute. Though some WPI students chose to go the solo route for their... Fast forward nearly 20 years and I am known by some colleagues as the “cross-team guy” at Addgene, an...

  6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...third chromosomes are the ones you’ll work with. If YFG is somehow on the Y or fourth chromosomes, you ...genome. They have four chromosomes: the X (and Y), second, third, and fourth chromosomes. Also, the fourth ...geneticists use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially are messed...that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature when setting...fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X...material are contributed by mother and father). While some mutations can remain stable as homozygous alleles... messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination...

  7. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... First, I will describe some of the many ways you can approach finding someone to give you advice and ...Second, I will offer some advice on how to “make the ask” once you have found someone you admire and want... you see someone who gives a great talk, ask them for advice on speaking. If you see someone with a great...connect you to. Sometimes, it is not who you know but who knows about you. I found some other great tips...circle. Asking someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor...relationships can last for an hour or for a year. Some click and some do not. These are all normal outcomes. If...whose career path you are interested in following? Someone you admire for their insight and way of expressing...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...targeted to lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis...degraded to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, ... the acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of ...negative/RFP positive) upon fusion of an autophagosome with a lysosome. An increase in autophagy leads to more... puncta, while a block at fusion of autophagosomes with lysosomes leads to an increase in yellow puncta...autophagy focused on counting the number of autophagosomes. This approach, however, has inherent limitations...Rosella is targeted to the vacuole in yeast or the lysosome in mammalian cells. This decrease in pH quenches...

  9. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...kids through some fun activities. If your school doesn’t have a Math & Science Night (or some equivalent...the teachers and sharing some slides on Twitter, where I was able to get some great feedback from teachers...Absolutely not. But seeing some students really get it, and trying to answer some of their great questions...contribute to my community. This past year, I spent some time thinking about how I could combine all of these...equivalent), here are some quick tips to get things going: If your PTA has a general meeting and a means to request... if activities are run by parents who can answer some of the surprisingly technical questions elementary... who think of themselves as “scientists.” We had some accountants running our math tables!  Be realistic...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...below.  Kids and adults alike enjoyed some friendly -or in some cases, serious-  competition with our .... No, we’re not all BFFs. Yes, sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather...there any have some fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene... is a higher likelihood that something will go awry. Don’t plan something that will involve a huge group...important parts of team buidling - they relieved some of the pressure of start up life and maintained ...manicured lawn of University Park at MIT.  I’m sure at some companies, employees wouldn’t be caught dead coming...us to work hard? Whatever it is, if you throw in some passion for our mission to share reagents with scientists...

  11. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...give someone a card they are more likely to remember you if you follow up and if you give someone your...is always visible. Some people learn names by seeing them. It is OK to ask someone to see their name tag... to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...up to someone much more senior than you, but you can safely seek out scientists your own age. Some will... you are on a deep level. Right away you have something in common. Namely, the scientific question you...know when you will meet a potential collaborator Some of these people you meet will review your papers... on a professional and personal level.  Here are some tips to get out of the rut of hanging out with only...

  12. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...the session, if someone comes up to my poster, I usually greet them and say something like “Let me know...Reach out if you’d like to meet someone and set aside a time to meet. Some conferences may also have a dedicated...with the policies before posting something on social media. If someone is presenting unpublished data they...include your university and your advisor’s name for some context and maybe common ground. The people you ...introduction. During the conference When you meet someone new at a conference, one of the first things you...several times at the conference, you might notice some trends. Were there parts of your elevator pitch ...arriving at the conference, plan out your agenda. Some conferences even have apps you can use to build ...

  13. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translational control in cellular physiology. Ribosome profiling Ribosome profiling, a biochemical method developed...mRNA bound-ribosomes in a cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the... translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating... instead takes advantage of the ribosome’s movement. When the ribosome moves along an mRNA, it displaces...translation inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5... especially during development or stress. With ribosome profiling, researchers have been able to study...extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted this technique...

  14. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...books for ABC. Some are easy reads, some are hard. Some I’ll never think about again, some I loved so much... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...most of the group really disliked, some that everyone loved, and some that ran the spectrum. I recently...Addgene. Since it’s National Book Month, we’ve asked some Addgenies to share their favorite books - both scientific...series that features a young female protagonist, some clever magic, and a satisfying conclusion? In this...age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly...selfish level, I wanted to be challenged to read some books outside of my comfort zone. I’d been in a ...

  15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...delivered and expressed in multiple somatic cells via various methods. Some of the more common delivery methods... epithelial cells from urine are some of the easily isolated somatic cells that have been reprogrammed...discuss the two major processes by which an adult somatic cell is converted to a different cell type for ...conversion happens via: ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which...Transdifferentiation In this process, differentiated adult somatic cells are converted to cells of a different lineage...stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to a pluripotent stage (iPSC...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...cytoplasmic contents. Autophagosomes that have fused with the vacuole or a lysosome are trickier to identify...localization or accumulation of pre-autophagosomal structures, autophagosomes and autophagic bodies in yeast...vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses with... targets cytosol and organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella...radio-labeled short-lived proteins primarily via proteasome degradation. Autophagy is then induced and quantified... autophagic structures based on morphology. Autophagosomes are relatively easy to identify: double-membraned...Atg8p (“p” stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for...

  17. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...the voice-over in videos), I sent some directions and (rules) to some volunteers, who filmed with their...setup to run some remote interviews, so the interviewee could see the interviewer. We’ve got some really cool...projects lined up, some that started production during the middle of lockdown, and some that will spin up... videos for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker...It’s really nice to have a scientist on-screen, somebody who really knows the protocol and can guide viewers...viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered, ...for longer than anticipated, we had to think of something else.   Figure 2: Ironing boards. Good ...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... However, some bicistronic vectors take advantage of an element called an Internal Ribosome Entry Site...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides, no consensus...the genes expressed from the same cassette is sometimes advantageous, particularly when only a portion...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA... can see that the IRES element acts as another ribosome recruitment site, thereby resulting in co-expression...have been discovered - many from viruses, but also some from cellular mRNAs. What they all have in common...found in bicistronic vectors; however, they do have some disadvantages. These elements are quite large (500...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...but some purification kits make it easier than others to prevent quality issues. For example, some kits...one you are most familiar with. And, hey — if something is working for you, then there is no reason to...an old standby to fall back to? We have outlined some important considerations to help you choose the ...need to recover and the level of quality required. Some applications require very little DNA and you can...source of waste in laboratories. To help combat this, some manufacturers have designed eco-friendly DNA purification...bottles or recycled cardboard packaging, there are some less obvious improvements that reduce the environmental...Separately Yes, buffers and columns sold separately Some, waste tubes sold separately No Other ...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...different. Some of these changes you may enjoy immediately and some of them may just take some getting used... counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues...settings: The sense of being the first to discover something. The thrill of having an experiment yield clear...if I was asked that question today, because I sometimes wish for a stretch of time that doesn’t involve...mentally challenging than lab work at times.” Sometimes a desk job is defined as sitting down at a computer... between tubes, look through microscopes, and sometimes you even wear a lab coat! These are the things...is that I’ve never been bored. There is always something to do and there is no thumb twiddling. But you...
Showing: 1 - 20 of 749 results