...Penguin Publishing Group. Day, F. (2019). Embrace your weird face your fears and unleash creativity. Gallery...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...can come any time. Meetings shouldn’t be the only place people share ideas. Ideas had over lunch are just.... Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand...backordered for months, we have to have plans in place to be able to continue our experiments. Thinking...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

...paragraph is the place to introduce yourself and your background. It’s a great place to state why you’...What is a cover letter for? A cover letter is a place to provide helpful context about your candidacy,...candidacy, beyond what is on your resume. It is a space for you to explain how your skills and experience match...candidate!  Additional uses The cover letter is a great place to proactively address questions about your application...you haven't talked about it yet, it's also a good place to state why you are interested in working for this...applicants. In short, while a cover letter can’t replace skills and experience, it can help the hiring manager...

...Bordetella spp., Caulobacter spp., Enterobacteriaceae, Gluconacetobacter xylinus, Haemophilus influenzae,...Gram-negative and Gram-positive  Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces...carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium...pSa, pR388 Gram-negative  Acinetobacter calcoaceticus, Aeromonas liquefaciens, A. salmonicida, Agrobacterium...campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1 ...Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous denitrificans, Pseudomonas...

...the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...modifications at DNA targets adjacent to NGG PAM sequences when used in place of wild-type SpCas9. The remaining...enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many do not... REPAIR (RNA Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to...

... Angela DePace has put personal development at the center of her lab (pictured above). DePace holds yearly...through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve around two simple...tone of the meeting is almost more important. As DePace says, science is full of “terrible perfectionists...have tangible benefits for both PI and trainee. DePace and her lab members have noticed that the meetings... Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016...

... in the extracellular domain which activates the native signaling cascade of the intracellular part of...ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive receptors...receptors, like mammalian rhodopsin, and the intracellular domain of an endogenous GPCR, such as adrenergic...signaling pathways? Here’s where photoswitchable intracellular proteins come into play. Photoswitches can be...state.      Figure 4: Example of an intracellular signaling pathway activated by an optogenetic...., & Cui, B. (2015). Optogenetic control of intracellular signaling pathways. Trends in Biotechnology,...

...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...science policy. Luckily, there are many mechanisms in place to ensure your effective networking---and they’re...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter- ...! Someone from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE... policy is so diverse, you can do it in so many places-- not just the government. When you talk to folks...technologies accessible to patients. And what better place to do that than at the nation’s largest integrated...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...

... entered human cells via hACE2, could not enter cells expressing murine ACE2 (Subbarao et al., 2006). ...SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example, is sustained...the University of Iowa (McCray et al., 2006), the hACE2 transgenic mice were designed after it was discovered...Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic hurdles...Look DC, Perlman S (2006) Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome...

... that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no.... IgD While IgD is coexpressed with IgM on the surface of most immature B cells, its function is unknown.... They are found bound to IgE receptors on the surface of basophils and mast cells, two types of white... the intervening DNA sequence is deleted, which places the variable region next to a different constant...

...target proteins. The surface-exposed loops of nanobodies seemed like a good place to put AsLOV2 because...inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry nanobody. Only insertion...-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs still bound...bound their target protein even when stuck to the surface of a bead.  Using OptoNBs to control cell signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...