...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...

...’re on a profile page. You might be looking at a list of suggested connections and you’ll see the boxes...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...optogenetics experiments in specific cell populations. Listen to Our Podcast Interview with Connie Cepko Combining...

...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...you will likely have is sifting through the long list of titles for something that is actually useful ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy...

...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...

...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...substrate (the histone-derived peptide) which can localise the construct specifically to the histone protein...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...

...more likely to view your proposals favorably. 5) Listen to your supervisor’s viewpoint and brainstorm about...with your supervisor, prioritize your action item list and set some approximate timelines. Be sure that...conflicts if you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for...

... advantages and disadvantages. Here is a table listing some of them: Characteristic ESC ASC Self-... For many years, ESCs or ASCs were the only realistic options for scientists working in regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...

...as dot blot, enzyme-linked immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (...blog post.  We generally do not recommend using ELISA-based titering assays, since those will quantify...Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...

...also been a great place to reach out to local establishments such as museums, professional organizations... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

... for you and your goals. Making a List of Schools Creating a list of schools is one of the first steps.... With virtual options, you can narrow down your list of schools by meeting with professors - a/k/a Principle...

...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

...cistron will start with the Proline.  The table below lists the four common 2A peptides employed by scientists...to express two or more genes, some of which are listed below. We should note that these vectors are primarily...gene or genes of interest. Some of the plasmids listed in the table above (and their related plasmids)...

.... What the BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier...like this conversation we are having now is in English, and then there are languages to communicate with.... The question is who owns languages? Who owns English? Well, everybody does; it's free to use and that...

...some accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...

...Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...

...Line Authentication Committee includes a database listing all cross contaminated or misidentified cell lines...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...

... a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest...mycoplasma detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

... All pCXLE vectors listed below in Table 1 are available at Addgene. Table 1: List of pCXLE episomal plasmids...removal of the residual viral particles and establishment of virus-free hiPSCs usually require about 5...

...or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, which will ...in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying appropriate...know very well; always ask for information and establish a connection with that person before ever asking...

...people or discussing science, it is a great way to establish yourself as a thought leader and have real conversations...on subreddits (r/”topic”) which are essentially lists of forums on particular topics. Volunteer moderators...moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping the quality...

...tables below listing some of the most common bacterial and mammalian promoters. These lists is by no means...on its orientation to your gene Although this list is a great place to start, the tables above do not...

...The following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016... for cell-binding abilities (6). This can be accomplished using a restriction enzyme with a short recognition...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

...were Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe...laboratories to start working on projects together. List of discussion topics at IMP In these sessions, mentors... Certified neurologist and multiple sclerosis specialist, currently President and owner of THiNKBrain,...

...contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of ... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...blogger! Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will...

...used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website. There...amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands using ... I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi:...