...getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology Resource Page Deposit...

...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...Simplifying Plant Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols...

...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...

... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

...Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan...al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in...al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source...

...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...the Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...

...American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3.... Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy...

...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...

...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond...Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our CRISPR resources and CRISPR for...

...identification of research reagents, several journals or organizations now recommend or require authors to use specific...well-established prefix resolution services such as Identifiers.org and N2T.net. To help authors comply with journal...Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will take you directly...

... paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q, Bourne PE...Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF... of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099  ...

...never forget the strawberries. Grand gestures are memorable! Attend events for one organization or go ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...

...action plans! As a project progresses it is easy to forgot the original plan. Write down your plans in a shared...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

...inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Richter MF, Zhao KT, Eton E...compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional resources ... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our...

...Proteins. Science, 244(4901), 182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z...Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., ... Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541  ...

...development. Vaccines. 7, 75 (2022). DOI: https://doi.org/10.1038/s41541-022-00503-y Gorry PR, McPhee DA...Transduct Target Ther 6, 53 (2021). DOI: https://doi.org/10.1038/s41392-021-00487-6 Additional Resources...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...

...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-19447-9 Hohlbein, J., Diederich...vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592-022-01602-3...

...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...

...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...

...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...strategies. IUBMB Life, 61: 222-228 (2009). https://doi.org/10.1002/iub.165  Additional resources on the Addgene...

... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...

...defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...

...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...