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We narrowed to 796 results for: arc

Showing: 451 - 500 of 796 results

  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced... Marcy Patrick...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...

  5. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...

  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...

  9. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...  Quintin Marcelino...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

  12. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...

  13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...

  16. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...

  17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

  20. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...

  21. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...interesting view into how rapid innovation affects the research community. I’ve likened it to the celebration ...currently at in the CRISPR software space. The CRISPR research community hasn't had a chance to develop a consensus...technology has been surrounded by a whirlwind of research activity and development since its inaugural year...

  22. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...say thank you to all the many scientists and researchers who deposit with us!  This year alone, we had...Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-...

  23. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...For example, if you’re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody...

  24. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...evidence that laughter really can be good for you. A search on PubMed for the word “laughter” turns up a few...evidence. (What can I say, we are scientists). Researchers in Taiwan showed that a laughter program significantly...

  25. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    [email protected]! Fun fact! Did you know you can search for plasmids from a publication by pasting the ...the paper's title into the Addgene website search bar?   We'll be celebrating all year long, so keep an ...

  26. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...synthetic biology. The guide is a curated list of research papers, blogs, free courses, YouTube channels,...

  28. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...communication is required to promote public support for research and to keep useful discoveries coming. At Addgene...are all run and organized by graduate student researchers. That’s right, #ActualLivingScientists, eager...

  31. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...landing page of the AAV Data Hub, you will be able to search for your favorite features, such as serotype, promoter...resource! We hope it helps you accelerate your research. Sincerely, Melina & The Addgene Team ...

  32. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...easier for you to find content relevant to your research and career goals, or to answer any questions you...

  33. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...they designed the protein computationally, the researchers expressed, purified, and characterized it, producing...introduction of de novo designed proteins also helped researchers understand more about the biophysics of protein...

  37. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...sequence of an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs...100% specific and can cut DNA sequences that researchers don’t intend it to. CRISPR cannot cut all sequences...

  38. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...stepped up to make COVID-19 reagents available to researchers across the globe very quickly. There are over...around the world quickly get started with COVID-19 research (check out this article in the GSA’s blog Genes...

  39. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, ...

  40. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Launchpad, and more Chapter 10 - Addgene's Future, the research team, and the importance of sharing information...received his PhD in Computer Science from MIT. His research focused on computer systems and networks. Benjie...

  41. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament...

  42. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...sciencedaily #instascience #ladies #research #cardiovascularresearch #parkinsonsdisease A post shared by...

  43. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...Request  has seen a fantastic response from the research community. Customers have taken advantage of the...look to see if these new preps might enhance your research!   Table 1: New ready-to-use viral vectors in...

  45. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...series will help you find new CRISPR tools for your research.     Improved nucleic acid sequence detection...human genome, these donor plasmids will help researchers map transcription factor localization and binding...

  46. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...Chiara DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR... travels with Blugene Blugene loves to visit researchers, so Outreach Scientist Jessica Welch showed some...

  48. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...mRNA binding factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA...published in The Journal of Biological Engineering in March is fused to a translational repressor. In principle...

  49. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...topic can be found on the UK Career Site for Researchers called Vitae. Vitae recently published a career...Download a summary PDF here or try out the online Researcher Development Framework Planner. For More Reading...

  50. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells because cells prepared...shock. Detailed protocols are available via Zymo Research. In brief, we grow our E. coli in LB to log phase...
Showing: 451 - 500 of 796 results