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Showing: 451 - 500 of 825 results
  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...
  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...10):2073. DOI: 10.3390/v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene...
  3. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline...
  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...
  5. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment...
  6. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles answering...reach out about writing a guest post (email news@addgene.org.) We are here to support and advance the scientific...
  7. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV ...
  8. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...are so ubiquitous in the lab that it is easy to forget that these enzymes naturally occur in bacteria ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular...
  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...
  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...
  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...types even when systemically administered in an organism. These features make AAV ideal for human therapies...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All...
  12. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...Download the Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here...
  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...
  14. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to...
  15. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...Introduction to Antibodies Additional resources on addgene.org  Antibody GuideRecombinant antibodies available...Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, N., Kim, Y., Lee, ...
  16. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...
  17. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...
  18. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ...
  19. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...components of biological research that are often forgotten or ignored. If we wish to conduct research that...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...
  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...
  21. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...work or volunteer for a science communication organisation like Building with Biology. Local environmental...Science Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...
  22. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...
  23. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies...
  24. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene ...Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources Sequence Analysis of a...
  25. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...antibody you used to assess the stripping, and don’t forget to re-block your membrane! Depending on the system...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video...
  26. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...in research. Nature 518, 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe...Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on ...
  27. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    .... Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007/s00018-019-03239-6 Additional resources...in our Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection...
  28. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering...
  29. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...
  30. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian, SP, et...Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer...
  31. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV in Addgene's Viral Service...
  32. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...used to conversing with other scientists that we forget that some of the terminology we use routinely isn...applications for funding from charities, commercial organizations, or universities may have someone senior from...
  33. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently Asked Questions Pages...
  34. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...
  35. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...
  36. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...
  37. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...frequently used species due to its use as a model organism in genetics and biochemistry. When using S. cerevisiae...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...
  38. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...Positive feedback is equally as important so don’t forget to recognize someone’s hard work and reward a job...Industry and Success in Management Resources at Addgene.org Addgene's Management Team ...
Showing: 451 - 500 of 825 results