...users. Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...worthwhile buying a dedicated machine. On the offline front, there is a Python script called SSFinder, but I...

...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

...ways that I could change the world around me. My strong interest in the intersections of media, art, and...forward but I have learned to push myself to the forefront, no longer allowing myself to just simply exist... an Addgenie. I thought about all that could go wrong with this internship but in the end it turned out...

...Moriarty, an Associate Professor at the University of Toronto, co-founded the COVID-19 Resources Canada site....groups have focused on iterating on methods for coronavirus research, sharing resources for COVID-19 research...our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene...

...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

...the workforce, but my main concerns were building strong connections with coworkers and doing something ...to engage with the work of others. Having an environment filled with people who are interested in understanding...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...

...Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first thing you'll...you need to know is your expression system or environment. The host organism will determine the type of...overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are...

...and chemogenetics communities to activate neurons and neuronal pathways using light and chemicals respectively...machines that can extend and retract over many microns in response to pH changes without the need for ...

...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

.... In neutral environments, dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless...mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences...

...requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits. ...information resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved...

...including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and ...and cell death post-transduction is low. Neuronal stem cells transduced with iTOP resulting in GFP...

...to graduate from university, many students are confronted with the question ‘what now?’ This is often a...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...exercise these skills outside of a formal work environment also shows that you are passionate and capable...

...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

...Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu...Infrastructure and a Vision for Future Enhancements. Frontiers in drug discovery, 2, 952326. https://doi.org/...

...PMC9015817.   Meet anti-GFP [N86/8R]! by: Ashley Waldron Anti-GFP [N86/38.1R] was among the first batch ...microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly...expansion microscopy as well as correlative light and electron microscopy and observed high fluorescence resilience...

...abstract comparing two fictional medical issues – chronic and acute “adeolitis” – received the best ratings...greater levels of science literacy are especially prone to this, possibly because a greater familiarity ...cloth menstrual pads while we talked about the environmental impact of disposable products. A great deal ...

...scientific community.Paratore: I’ve certainly had a front row seat watching the international growth through...whether that be academically or on the employment front. Paratore: I work at Tufts Health Plan and my role...operations. We’re looking for those “what could go wrong scenarios” and making sure we have adequate process...

..., which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the protein...of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary. This....com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

...to create a more productive and cohesive team environment. Neglecting communication skillsClear communication... great "baby project," offering a supportive environment to build my management skills. Finally, developing...effectively and foster a cohesive, productive environment. For example, in my current role managing a cross-functional...