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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 367 results for: his

Showing: 181 - 200 of 367 results

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based strategy...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...of CRISPR in 2013 (Cong et al., 2013)! Haoyi got his first exposure to CRISPR when he used this new technology...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...N terminal); 6xHis (N terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal);...pET-Cas9-NLS-6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... still infect the researcher and insert into her/his genome - this is a biosafety risk Infects dividing...

  11. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...likely to lead to scores. That player doesn’t trust his teammates; the teammates don’t trust him. This team...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

  13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... Associate Product Marketing Manager at Promega. His work focuses on commercializing their cell and protein...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ...

  18. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...Additionally, I would like to thank Le Cong for all his hard work creating amazing resources for the scientific...your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong et. al (2013...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...large-scale expression screen that can be used to generate His-tagged human kinase constructs that express well ... Saccharomyces strains by compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine...epigenetic modifiers like DNA methyltransferases or histone deacetylases. Unfortunately, these inhibitors are...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ..., Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase reporter gene...respond to their environment - typically through a histidine kinase, which triggers phosphorylation of the ...
Showing: 181 - 200 of 367 results