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Showing: 181 - 200 of 809 results
  1. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...form of figures/schematics or tables. And don’t forget to include citations so that people can go back...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info...
  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances...gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene...
  3. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our...
  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang... Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang Q, Xu...Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional resources...all articles about microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids ...
  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...
  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...of paper in notebooks, and hoping that we don’t forget to write things down when doing scientific research...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...
  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...
  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Alberta in Edmonton, Canada, to the University of Georgia, USA) has developed that allows for a phage genome...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...lab during its recent move to the University of Georgia). She enjoys pondering how phages get through the...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...
  9. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully, I’ve ...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...
  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the...Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar A ...out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez...Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871 Pray, L. (2008) Transposons... for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the...
  11. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... Volunteering at a Professional Organization Resources on Addgene.org Check out Career's at Addgene ...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...
  12. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...untagged counterparts. These findings motivated Georgieva et al. to develop new tags with defined 3D structures...aggregates. Using budding yeast as a model system, Georgieva et al. examined the effects of GFP fusions to .... To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28...epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...
  13. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages... about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring artists who are... our transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career...
  14. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    .... Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M.W., Nguyen...immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen...system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...
  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner...vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M., Schütze...all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources on the...Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...
  16. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...
  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska...and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al ...SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the...Against Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody...
  18. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...
  19. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman...Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources on ...parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...
  20. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, ...In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional resources... precise targeted manipulation Resources on Addgene.org Browse all viral vectors available at Addgene...
Showing: 181 - 200 of 809 results