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We narrowed to 813 results for: org

Showing: 181 - 200 of 813 results

  1. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, Rego M, Bourges...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011...NGS for Plasmid Quality Control Resources on Addgene.org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus...

  2. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein, J., Emrich...cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais,...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein, B., &...United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He,...Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G...microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...has revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided...screens required to identify correctly modified organisms has limited routine use of this technology. To...tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University of Utah developed SapTrap...conditionally knock down proteins in a variety of model organisms. As long as it was expressed in a cell that also...

  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi.... The EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar, G., Giraldo...Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade, J. E.,...Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson,.... The EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources at Addgene ...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang... Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang Q, Xu...Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional resources...all articles about microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids ...

  7. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances...gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...form of figures/schematics or tables. And don’t forget to include citations so that people can go back...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...of paper in notebooks, and hoping that we don’t forget to write things down when doing scientific research...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...

  13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Alberta in Edmonton, Canada, to the University of Georgia, USA) has developed that allows for a phage genome...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...lab during its recent move to the University of Georgia). She enjoys pondering how phages get through the...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...

  14. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully, I’ve ...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the...Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar A ...out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez...Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871 Pray, L. (2008) Transposons... for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the...

  16. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... Volunteering at a Professional Organization Resources on Addgene.org Check out Career's at Addgene ...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...

  17. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...untagged counterparts. These findings motivated Georgieva et al. to develop new tags with defined 3D structures...aggregates. Using budding yeast as a model system, Georgieva et al. examined the effects of GFP fusions to .... To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28...epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...

  18. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages... about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring artists who are... our transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career...

  19. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    .... Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M.W., Nguyen...immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen...system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

  20. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner...vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M., Schütze...all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources on the...Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...
Showing: 181 - 200 of 813 results