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  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope ...

  3. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...experiences, but the increased binding can mask the epitopes antibodies recognize. It is therefore best to ...for one that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit... be transduced equally well by AAV-retrograde serotypes (Skorput, 2022, Albaugh, 2020, Tervo, 2016).  ...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Tools blog can help you learn about the different types of viruses and pick one for your experiment. Research... of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral...

  6. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... creating and sharing knowledge, related to the types of materials we help distribute and the research...Then we format, do a final review to catch those pesky typos, and hit publish. Making content is an iterative...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...and gamma (Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11...GPCRs show a propensity to associate with certain subtypes, although most are able to signal through more...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose...drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes and metabolism...

  9. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...multiplexing and can be used in a variety of assay types. Be sure to check the spectral properties of the... et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al....

  10. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...amount of working memory resources. There are three types of cognitive load: intrinsic cognitive load is the...organizing and connecting information and a logic to the types and timing of questions that are asked. Many things...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibodies are antibodies generated against antibody isotypes from a specific species, such as the rabbit IgG...primary antibodies are different species and/or isotypes. For highly multiplexed assays, using secondary...

  14. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell an engineer... barcoded (this is pretty Addgene specific) The types or purposes of each given container, when applicable...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...embryonic stem cells, and/or tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox... that are active only in certain cell or tissue types, during certain stages of development, or by making...

  16. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...then injected into a blastocyst. There are several types of genetically engineered mice: transgenic mice,...in part 2 of this blog series) controls the cell types in which the conditional recombination can occur...

  17. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...addition, scientists have engineered entirely new types of plasmids without clear analogues in nature. For...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...studies in neuroscience where specific tissues, cell types, or even subcellular regions of a neuron need to...expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase systems...

  19. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...turns green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte...well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein ...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...a paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following...fields of research they support. Monitoring these types of accounts can help you find papers related to ...
Showing: 181 - 200 of 429 results