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We narrowed to 17 results for: pes

Showing: 1 - 17 of 17 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...other than PES. AAV particles stick to many other surfaces, but do not stick to PES. Using a PES filter will...polyethersulfone (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...supernatant as follows: Filter through a 0.45 μm PES membrane. Add 40% PEG such that the final PEG concentration...sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone SH30237.01 (optional) L-alanyl-L-glutamine..., 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose. 1 mg/mL polyethylenimine...Often AAV2 are harvested at 48–72 h, while other serotypes are harvested at 96–120 h. Optional: perform a...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...filter, VWR 431229 0.22 µm PES filtering system, 1000 mL, VWR 431098 0.45 µm PES filtering system, 500 mL...2 mg/mL) Mix well and sterilize through a 0.2 µm PES syringe filter. Aliquot and freeze upright at -20... cells. Filter the supernatant through a 0.45 µm PES filter. Add 1X protease inhibitor cocktail to the...

  3. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Microcentrifuge tubes, VWR 89126-724 Filtering system 0.2 µm PES, VWR 431098 Sodium phosphate monobasic monohydrate...T2819-1L 250 mL sterile bottle, VWR 430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR...aprotinin Mix well and sterilize through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure...

  4. Lentivirus Production

    Type
    Protocol
    ... acid Sodium hydroxide 0.22 μm polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging...harvesting. Filter supernatant through a 0.45 μm PES filter. The viral supernatant can be stored at 4 ...

  5. General Transfection

    Type
    Protocol
    ... acid Sodium hydroxide 0.22 μm polyethersulfone (PES) filter Syringes for filtering Reagent Preparation...

  6. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...conditioned medium by filtration through a 0.45 µm PES filter or centrifugation at >500 x g for 5 minutes...concentrations, may be optimal for different cell types. Before starting this procedure, be sure that the...the lines for transgene expression and/or other phenotypes. For example, perform Western blotting to screen...

  7. Using a Light Microscope Protocol

    Type
    Protocol
    ...dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable microscopes that...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...accessible) to massive transmission electron microscopes that allow us to see cellular components with...protocol, we'll focus on one of the most common types that you're likely to encounter: the compound light...light microscope. As the name suggests, light microscopes take advantage of the physical properties of ...you may wish to record your observations. Some microscopes have digital cameras attached to them that allow...capture images directly to a computer, but many microscopes do not and you may have to dust off your drawing...

  8. Centrifugation

    Type
    Protocol
    ...found on almost any centrifuge. Types and Parts There are several types of centrifuges: Mini or Tabletop...tube. You will likely encounter several different types of centrifuges that can accommodate different sized... are typically lower than some other centrifuge types. Floor Centrifuges sit on the floor and can be about...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... I.1 Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-...particles can efficiently infect a broad range of cell types, including both dividing and non-dividing cells....stuffer insert is shown in all capital letters. I.2. Recipes Luria Broth Agar (LB agar) + antibiotic Per 40 ...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...scale. This will provide more accuracy as these types of scales display more decimal places. Pro-Tip If...

  11. Water Bath Protocol

    Type
    Protocol
    ..., and thaw cell lines. There are many different types of water baths that exist as scientists work with...

  13. Immunocytochemistry

    Type
    Protocol
    ...Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes Before Starting...

  14. Pipetting Protocol

    Type
    Protocol
    ... Pipette Tips Although there are many different types of pipettes that have different mechanisms to disperse...

  15. Pouring LB Agar Plates

    Type
    Protocol
    ...should kill most potential contaminants, certain types of spores will stubbornly survive even under the...

  16. AAV ddPCR Titration

    Type
    Protocol
    ...Supermix for Probes), the target names and target types. When the plate layout is complete, select 'Run'...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Supermix for Probes), the target names and target types. When the plate layout is complete, select 'Run'...
Showing: 1 - 17 of 17 results