Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 12 results for: pes

Showing: 1 - 12 of 12 results

  1. CRISPR Guide

    Type
    Guide
    ...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced... Overview of prime editing Popular prime editing (PE) tools include: PE2 - improved RT enzyme efficiency...employed to knock out target genes in various cell types and organisms, but modifications to various Cas ...are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a and...about 8 bp, specified by a gRNA. There are two main types of base edits: Base transitions - edit to same classification...then carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...

  2. Antibody Guide

    Type
    Guide
    ...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ in their...their tropism — that is, the types of cells they infect. Serotypes are determined by naturally occurring...tissue tropism displayed by different AAV serotypes. Pseudotypes Pseudotyping is the process of producing...capsids and genomes from different viral serotypes. Pseudotypes are generally denoted using two numbers... of cell types in vivo. AAV-DJ has been optimized further for specific cell and tissue types, like AAV-DJ8...vectors to ~2.4 kb. Figure 4: Examples of AAV serotypes, pseudotypes, hybrid capsids, and capsids created through...: AAV Vectors Vector Production Integration AAV Types Common Uses Safety Resources and References Plasmid...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This means... associated with many serious illnesses. Common types include murine leukemia virus (MLV), murine sarcoma...them useful research tools in a variety of cell types and models, such as stable long-term expression ...lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses... a wide range of infectivity for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids...to pseudotype gamma-retrovirus using different envelopes to modify tropism, as the env gene can easily ... of cancer. Pseudotyping Tropism dictates which types of host cells the gamma-retroviral vector will infect...

  5. Adenovirus Guide

    Type
    Guide
    ...transgene expression. Adenoviral Types Serotypes More than 60 serotypes have been identified within human...capsids and genomes from different viral serotypes. Pseudotypes are generally denoted using two numbers...Vectors Vector Production Integration Adenoviral Types Common Uses Safety Resources and References Plasmid...classified into seven species (A to G). Different serotypes exhibit varying tissue tropisms, meaning they ...to their high gene transfer efficiency. Other serotypes like Ad26 and Ad35 are also of interest for their...avoid vector-neutralizing pre-existing immunity. Pseudotypes Pseudotyping is the process of producing viral...with the low immunogenicity of the Ad35 and Ad26 serotypes. Common Uses of Adenoviral Vectors Although recombinant...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This means...disease and occurs in many species. Some common types of lentivirus include Human immunodeficiency virus...them useful research tools in a variety of cell types and models. Lentiviral vectors can target non-dividing...lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses... a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors have gone... examples). Pseudotyping Tropism dictates which types of host cells the lentiviral vector will infect....has a wide range of infectivity across many cell types and enhances the stability of the viral particles...

  7. Chemogenetics Guide

    Type
    Guide
    ...constructs and the cell types in which they drive expression. Different AAV serotypes can be used to target...chemogenetic receptors. There are several different types of DREADDs that can be broadly classified by the...may need to be targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific...cells of interest. In addition, different AAV serotypes have varying degrees of viral spread within injected... be considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used...express the chemogenetic receptor in unwanted cell types. Generating a FLEx switch to control expression ...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ...can be divided into two types: positive screens and negative screens. Both types of screen employ a selection...how to amplify and use a pooled library; and what types of screens can be done with a pooled library. Science...Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to Pooled Libraries...can also negatively affect data reproducibility. Types of Pooled Library Screens In pooled library screens...

  9. Plan Your Experiment

    Type
    Guide
    ...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other options. Browse...with, and are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral...cells. You can direct expression to specific cell types by using cell-specific promoters, which is useful...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

  10. Molecular Biology Reference

    Type
    Guide
    ...Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains for propagating plasmids...biologist’s toolbox. To learn more about different types of cloning methods check out our guide on molecular...promoters, small RNAs, or other genetic elements. Types of Plasmids Plasmids are versitile and can be used... used in the lab. Below are some common plasmid types: Cloning Plasmids - Used to facilitate the cloning...Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc.), depending largely...

  11. Promoters

    Type
    Guide
    ...the lac promoter Hybrid promoter of lac and trp Types of RNA Polymerases Promoters control the binding...initiate the transcription of genes. There are three types of RNA polymerases that all transcribe different...

  12. Science Guides

    Type
    Guide
    ...educational guides to learn more about the different types of viruses that are commonly used in research, including...
Showing: 1 - 12 of 12 results