...independently of DNA synthesis. McCarty DM, Monahan PE, Samulski RJ. Gene Ther. 2001 Aug;8(16):1248-54. ...cells and is a key advantage of rAAV. AAV Serotypes Eleven serotypes of AAV have thus far been identified,...commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making...transducing specific cell types. The chart below gives a summary of the tropism of AAV serotypes, indicating the...capsid and genome from different viral serotypes. These serotypes are denoted using a slash, so that AAV2...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...you about AAV components, serotypes, pseudotyping, and more. Science...

...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced... Overview of prime editing Popular prime editing (PE) tools include: PE2 - improved RT enzyme efficiency...employed to knock out target genes in various cell types and organisms, but modifications to various Cas ...are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a and...about 8 bp, specified by a gRNA. There are two main types of base edits: Base transitions - edit to same classification...then carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...

...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

...constructs and the cell types in which they drive expression. Different AAV serotypes can be used to target...chemogenetic receptors. There are several different types of DREADDs that can be broadly classified by the...may need to be targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific...cells of interest. In addition, different AAV serotypes have varying degrees of viral spread within injected... be considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used...express the chemogenetic receptor in unwanted cell types. Generating a FLEx switch to control expression ...

...dividing cell types whereas standard retroviruses can only infect mitotically active cell types. This means... and cell types. For more information, see the Cronin, et al. article on different envelopes and their...are generalized and appropriate for varied cell types and systems. When planning your experiment, the ...lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and standard...

...can be divided into two types: positive screens and negative screens. Both types of screen employ a selection...how to amplify and use a pooled library; and what types of screens can be done with a pooled library. Science...Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to Pooled Libraries...can also negatively affect data reproducibility. Types of Pooled Library Screens In pooled library screens...

...Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains for propagating plasmids...biologist’s toolbox. To learn more about different types of cloning methods check out our guide on molecular...promoters, small RNAs, or other genetic elements. Types of Plasmids Plasmids are versitile and can be used... used in the lab. Below are some common plasmid types: Cloning Plasmids - Used to facilitate the cloning...Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc.), depending largely...

...in your target cells. For easy-to-transfect cell types (e.g. HEK293 cells), transfection with standard ...cell lines Useful for difficult to transfect cell types, can also be used in vivo A common choice for conducting...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

...transcripts. There are 57 accepted human adenovirus types, and most adenoviral vectors are based on Ad5. Ad5...adenoviral vector constructs. The system consists of two types of plasmids: shuttle (or transfer) vectors and adenoviral...

... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope...

...the lac promoter Hybrid promoter of lac and trp Types of RNA Polymerases Promoters control the binding...initiate the transcription of genes. There are three types of RNA polymerases that all transcribe different...

...educational guides to learn more about the different types of viruses that are commonly used in research, including...