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We narrowed to 53 results for: pes

Showing: 1 - 20 of 53 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...(M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components have helped to improve...exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available: PE2 - introduction...degradation For more in-depth information on these PE tools, check out our prime editing blog post . Need...Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 176901 U6-pe RNA-H1-nick sgRNA-mCherry Mammalian,...

  2. Viral Production

    Type
    Collection
    ...by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen...

  3. CRISPR Guide

    Type
    Collection
    ...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced... Overview of prime editing Popular prime editing (PE) tools include: PE2 - improved RT enzyme efficiency...employed to knock out target genes in various cell types and organisms, but modifications to various Cas ...are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a and...about 8 bp, specified by a gRNA. There are two main types of base edits: Base transitions - edit to same classification...then carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...cloning of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology...plasmids may be successfully adapted to numerous cell types using preferred culture conditions and transfection...

  5. Antibody Guide

    Type
    Collection
    ...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

  6. Viral Vectors

    Type
    Collection
    ...Use this guide to learn more about the different types of viruses that are commonly used in research, and...find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses... standard cloning methods). There are different types of viruses that are commonly used for research, ...studies because of its low immunogenicity. Different types of viruses also vary in the composition of their...a brief overview of the properties of different types of viruses. More detailed information can be found...provides basic guidelines for working with various types of viruses; however, these guidelines may differ...

  7. AAV Viral Preps

    Type
    Collection
    ...the eye and other tissues All Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...repository and request packaging in one of seven serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11, or AAVrg... of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde...AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed on the Function section...

  8. Serotype Testing AAV

    Type
    Collection
    ...viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5, AAV6, ...can be used to compare the tropism of different serotypes. In addition to the standard 100 µL sample size...be used to validate the performance of various serotypes before conducting larger scale experiments. For...#37825) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...#50465) Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...many different cell types in the body during development. Unlike other cell types, each stem cell has ...stem cells, can differentiate into multiple cell types, but are typically limited to related cell lineages...somatic stem cells or fully differentiated cell types. Browse Addgene's collection of Differentiation...Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell...Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6. Buganim...

  10. Plan Your Experiment

    Type
    Collection
    ...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...kidney 293 (HEK293) cells. For more difficult cell types, you may need to look into other options. Browse...with, and are good choices for more difficult cell types like primary cells. For CRISPR delivery, lentiviral...cells. You can direct expression to specific cell types by using cell-specific promoters, which is useful...edit. CRISPR editing produces several possible genotypes within the resulting cell population. Some cells...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex Schier Lab Other ...ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically...carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...brain parenchyma or subretinal space Serotypes The following serotypes were developed in Arun Srivastava’...Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service...plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid name ...

  13. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based ... the targeting capability of Cas9 to make other types of modifications at specific loci. Activate or Repress...

  14. Bacterial Expression Systems

    Type
    Collection
    ...Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia...to create, store, and replicate plasmids of all types, but beyond that, researchers also use bacteria ...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...

  15. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ...variation affects genome function to influence phenotypes....predict, characterize, and map how genome function shapes phenotype, and how these processes are affected...impact of genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF...

  16. Plasmid Collections

    Type
    Collection
    ... use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering ...here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A ...here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  17. Optogenetics Plasmid Collection

    Type
    Collection
    ...different types of proteins. For a full description (including peak response spectra) of different types of ...track its expression and localization. Many other types of proteins can also be controlled by light, generally...

  18. AAV Packaged on Request

    Type
    Collection
    ... that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...plasmids to be packaged in their choice of seven serotypes, resulting in over 28,000 possible choices! By...only package plasmids in our collection in the serotypes listed here. Details Scale and Titer Packaged ...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This is due to the...components Common uses of AAV Viral integration AAV serotypes Pseudotyping Want to save time? Check out our ...E2A and VA James M. Wilson RepCap Plasmids for Serotypes Available as a Viral Vector Packaging Service ...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru laboratory and the .... To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech...Boyden Citation Information When using the Caltech serotypes in future publications, please acknowledge Viviana...
Showing: 1 - 20 of 53 results