...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ... welcoming, inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or...supportive Board, we have a robust committee process in place and continue to incorporate additional Board best... hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive culture, we ...

...target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...starting. We recommend calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and...some final adjustments, this is generally a good place to start. If you don’t anticipate that you will ...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

...other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, ...they are not currently provided. Eye protection or face shields are required whenever there’s a splash or... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated after use,...basically, you’ll need to get trained in each new place (and pay attention to the trainings!), even if you...

...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine... GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...research, and prevent technological advances from exacerbating existing inequalities and injustices. The Personal...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....quickly into sensitive topics and uncomfortable places, from sticky ethical dilemmas to dark episodes ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

... by ultracentrifugation (75,000 - 225,000 g for 1.5 – 4 hours at 4 °C). After ultracentrifugation, virus...vectors) you can concentrate your virus by ultracentrifugation of the viral stock, followed by resuspension...packaging cell debris before proceeding to ultracentrifugation to avoid contaminating your transduction ... to concentrate a virus solution without ultracentrifugation, you can reduce the volume of cell culture...exchanging the medium after transfecting cells, replace the medium with half the volume typically used ...

...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

... they not only traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible...ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide emerges...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...

... Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous...targeting takes advantage of this natural process to replace a targeted genetic locus with homologous sequence...gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus disrupted...recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted gene is disrupted ...normally because one of its exons has simply been replaced with the same sequence flanked by LoxP sites while... while the resistance cassette has been placed into an intron.    Figure 4: A targeting vector...

...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...