We narrowed to 762 results for: SOM

...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...

...research done in our depositor labs. Some are written by Addgenies and some are written by the depositors themselves...favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed...

...the loading and unloading of the column and give some tips for performing this purification.  Check out...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to... new to tissue culture, check out this video for some tips and best practices for getting started with...

...these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped...your science along? We’ll also take a look back on some fascinating Addgene history that you might not have... into 2021. Check out the infographic below for some Addgene depositor fun facts for a quick way to grasp...

...content teams have made some changes to the look and feel of addgene.org. Some you’ll notice right away...Starting today, you may notice some changes to our website! Part of sharing science and making access...

...transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9-2A and the Puromycin resistance...the name? Not included in this plasmid's name are some additional features. The first and most imporant...Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian...

...colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation... and also serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great... enough people will be vaccinated and we’ll have some herd immunity, which raises questions like “How ...

... perturbations. So, while some might argue that the only way to do something is to do it huge, there are...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...

...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...competitions in the future, and hopefully include some of our favorite winning recipes. Hack Day lunches...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...

...Plasmid Collection for “Functional Annotation of Somatic Mutations in Cancer.” Of all the mutations and ...how a given gene is involved in a complex pathway. Some of the most heavily-studied genes in cancer research...Kwok-Shing, et al. "Systematic functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): ...

... is very specific. Some infect only one or a few species of bacteria and sometimes, only a subset of strains... cry wolf to the phage community until we have someone that really needs it, but then it might to be too...

... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...and I believe it could have made the difference in some areas of conservation.” Appreciating science as ...focused his x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of ...

... was set aside for the event. As guests arrived, some gravitated towards the sushi and drinks, while others...Addgene family. It was great to see the faces of some of our depositing scientists who weren’t able to... roam the museum. I even posed for pictures with some Addgenies. As the gala came to an end, it was clear...

...was useful to me as some days it was not as easy to commute as others. I know some fear that remote work... Working a 9-5 As it turned out, Addgene uses something called “core hours”. Core hours consist of the...

...support team available whenever you need them, but sometimes that’s not enough. With your busy schedule, you...prefer to troubleshoot yourself without contacting someone else and waiting for an answer. Luckily, there’...easier than ever to get the support you need. If for some reason you can’t find the answer that you’re looking...

...excitement, in a position. Everyone prefers to hire someone that will be excited to be there. If you are appying..., you will need to be more directed and target someone in a specific department and/or location. The more...asking for help. The worst thing that can happen is someone will say no, but if you don't ask, you will never...

...We also updated some our most popular CRISPR blogs to keep them current. Here are some of the changes ...’ve also added four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing...

...and everything in between – now all you need are some of your favorite proteins purified to carry out ...used for both direct and indirect visualization. Some of these tags can also be used for protein purification...purification, just as affinity tags can sometimes be repurposed for visualization. However, due to the nature...

... labor-intensive project; scripts can be used in some cases, but much of this work has required manually...the graduate student down the hall who got it from some technician downstairs who got it from who knows ...submissions from scientists all over globe, with some surprising, and important, findings being shared...

...impression I got from the La Vida Scholars tour. After some time had passed, I reached out to Amy with exciting...the Summer Intern Poster Presentation.  As someone who had limited experience when it came to lab ...full potential as a scientist. But I expected something wrong. My capstone project was based on plasmid...

...Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans through...and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional antibodies...libraries to generate new sdAbs. There has also been some interesting work done developing transgenic mice...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...

...either. But with a little Googling and some trial and error, I found some free design software that let me ...for drawing figures with lots of elements. It took some playing around to figure out all the settings and...

... Our original policy was straightforward, but cumbersome: depositors had to email us with permission to...distribution, and we’ve found that this step was sometimes unclear or missed altogether. Emailing Addgene...isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’re happy to ...

...sequence alteration. Molecular cloning techniques are some of the most widely used techniques in the biological...efficiency and simpler procedures have been developed. Some of these include, TA cloning, ligation independent... step by step procedure of FastCloning, here are some tips for high efficiency cloning with this technique...

...with the academic enterprise or that there weren’t some pretty hefty bumps during my graduate training. ...path to science communication This may come as somewhat of surprise to many of my high school friends....being an Outreach Scientist at Addgene has been awesome. The process of writing, editing, and publishing...

...good. Don’t pretend something went well when it didn’t… but don’t “punish” someone for a past infraction...positive feedback when it is merited. Surprisingly, some people find this hard so get used to it by making...

...described below some of the most frequently requested vectors in our repository and will discuss some of the features...approaches to studying YGOI's function in worms. Some of the more popular studies involve using a reporter...

...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time... genetics during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery from... many interactions with Addgene over the years. “Some of the walls we’ve built create inefficiencies that...

...genome inside a protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...the cell like baby spiders from an egg sac. (Okay, some enveloped viruses bud out, instead of lysing, but...