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  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...GC’ sequence context. If this cannot be avoided, select the target AID version. Expect very low to nil ...

  3. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid...

  5. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...will fight more adamantly for their solution), selecting a devil’s advocate is a good option for any meeting...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...Addgene offers vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...above. There’s many variations in how one can gate, select, and analyze data. But now that you can read a ...

  17. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...information for your senators all you need to do is select your state. State Legislatures: To find the website...

  19. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...from the parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...still be used to screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels ...

  22. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of interest...

  24. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...101: Choosing the Right Isotype Antibodies 101: Selecting the Right Antibody Resources on Addgene.org Addgene's...

  25. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid Multimers...

  27. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... other materials pass through; and, finally, selectively elute the plasmid DNA from the column using a...

  29. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...your general scientific expertise and then once selected, you can pivot to a completely new field without...
Showing: 301 - 330 of 344 results