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Showing: 301 - 330 of 814 results
  1. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...the Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...
  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...action plans! As a project progresses it is easy to forgot the original plan. Write down your plans in a shared...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...
  4. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...
  5. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...
  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...
  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...Proteins. Science, 244(4901), 182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z...Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., ... Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541  ...
  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...development. Vaccines. 7, 75 (2022). DOI: https://doi.org/10.1038/s41541-022-00503-y Gorry PR, McPhee DA...Transduct Target Ther 6, 53 (2021). DOI: https://doi.org/10.1038/s41392-021-00487-6 Additional Resources...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...
  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-19447-9 Hohlbein, J., Diederich...vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592-022-01602-3...
  10. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Richter MF, Zhao KT, Eton E...compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional resources ... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our...
  11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...
  12. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...
  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...
  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...
  15. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...
  16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists to use ...optogenetics. Nat Methods 16:1176–1184 . https://doi.org/10.1038/s41592-019-0583-8 Additional resources ...chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene...
  17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...
  18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...
  19. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly go over it now'?...Chinese undergrads. You get nervous and freeze, or forget a point, or talk extremely quickly. You realize...happens to other people, it’s normal and quickly forgotten, and when it happens to you, it’s noteworthy and...
  20. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038/s41580-021-00430-1 More resources on the...
  21. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...
  22. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019...Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101 Additional resources on the...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection...
  23. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...
  24. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...Using Barcodes to Track Samples Resources at Addgene.org Find protocols for standard molecular biology...
  25. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a..., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting cell cycle...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR corner...
  26. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA...therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional resources ...CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our CRISPR guide...
  27. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519 More resources from ...
  28. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from...
  29. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...accurately.   Priode and Haynes, protocols.io. 2021. dx.doi.org/10.17504/protocols.io.brgcm3sw     New system... cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for the...studies.    Duong et al., Genetics 2021. https://doi.org/10.1093/genetics/iyab006       The CRISPR corner...
Showing: 301 - 330 of 814 results