... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...

...inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists to use ...optogenetics. Nat Methods 16:1176–1184 . https://doi.org/10.1038/s41592-019-0583-8 Additional resources ...chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene...

...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...

... antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019...Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101 Additional resources on the...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection...

... Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly go over it now'?...Chinese undergrads. You get nervous and freeze, or forget a point, or talk extremely quickly. You realize...happens to other people, it’s normal and quickly forgotten, and when it happens to you, it’s noteworthy and...

...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038/s41580-021-00430-1 More resources on the...

... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...

...   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a..., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting cell cycle...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR corner...

...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA...therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional resources ...CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our CRISPR guide...

...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038/s41593-019-0431-2  Neff, E.P. An AAV library...macaque, and man. Lab Anim 48, 264 (2019). https://doi.org/10.1038/s41684-019-0380-0...

...accurately.   Priode and Haynes, protocols.io. 2021. dx.doi.org/10.17504/protocols.io.brgcm3sw     New system... cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for the...studies.    Duong et al., Genetics 2021. https://doi.org/10.1093/genetics/iyab006       The CRISPR corner...

...Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from...

...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519 More resources from ...

...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...Using Barcodes to Track Samples Resources at Addgene.org Find protocols for standard molecular biology...

...English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator... at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...CRISPR Michael Terns from of the University of Georgia studies the CRISPR/Cas system as it relates to ...

... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...

...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

... of your gene’s functional biology in a living organism. This is the first post in a three-part series...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

...is “…where molecules become real”.  We sometimes forget that the models we create are around 30 million...reticulum. And graduate students, momentarily forgetting that the physical model is based on their own...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...

... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...in the comments section below or contact blog@addgene.org if you'd like to write a more detailed post ...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

..., enter the common or scientific name into the Organism box. This small piece of information can significantly... of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene...

...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from excitation...

... (e.g. Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to ...Contact our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for ...

...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...101: Green Fluorescent Protein Resources on Addgene.org Browse our Fluorescent Protein Collection Find...

...Worried about your budget? Email janeliafluor@janelia.hhmi.org to try out the JF dyes. Acknowledgements ...2015): 917-923. PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside...Fluorescent Protein Should I Use? Resources on Addgene.org Generate Fluorescent Protein Fusions Request...