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Showing: 501 - 550 of 796 results
  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology...GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning ...
  2. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...will help you find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors...lentivirus-like particle. Read the paper in Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine...
  3. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Addgene Director of Biology, Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights... Addgene's Cancer Collection Pages Connect the Research Community Grad School Advice Part 1: Picking a...
  4. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...you might expect with any nascent technology, researchers are having trouble getting NgAgo genome editing...successes, and to find out what delivery techniques researchers are using. The detailed results of his survey...
  5. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...interesting work. Consider this to be like any other research project. You’ve met someone whose career interests...Informational Interview is a great next step in your research. What an informational interview is not If ...
  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... cells, and it may find wide use in biological research. Photoconvertible fluorescent proteins Newly...
  7. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... purification specialists recommend doing some research, notably determining the subcellular localization...for cloning (see schematic below), enabling a researcher to test multiple tags for one protein quickly...
  8. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...) to make it easier to know where to start your search. Then identify the most frequently used pages in...checklists that link to other notes, and use the search bar to quickly find what you’re looking for. Tip...
  9. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... a scientist). Profiles and Twitter lists were searched for scientific titles generated from the United...Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls. 5. The #1 (re)tweeted...
  10. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...representing imaging from 47 different tissues. Researchers upload and share both their successes (recommended...detergents that they used in their experiments. Researchers can then visit the IBEX imaging community discussion...
  11. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...tissue types, and across species, it enables researchers to select and generate physiologically relevant... it’s an exciting time to be in the biomedical research field! References and Resources References Fellmann...
  12. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...     HcKCRs represent a long-sought tool for researchers to control K+ gradients and can be used as a ...of the most commonly used protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up ...
  13. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...By mutating one of two Cas9 nuclease domains, researchers created the CRISPR nickase. Nickases create a...editing. For more information and a link to the archived webinar, click here. We would like to thank IDT...
  14. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving field, and...overview of the development of CRISPR into a varied research toolbox, as well as additional resources to help...
  15. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...constitute foundational elements of biomedical research. Traditional restriction cloning is one of the...Resources on Addgene.org Find Empty Vectors for Your Research Find Additional Protocols Browse Our Special Collections...
  16. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ... and current, trending topics in their area of research. The society also organises PhD information events...with, learn from, and mentor while pursuing your research interests. Scientific societies like those mentioned...
  17. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...satisfaction are a bit surprising. There is a lot of research in this area and studies overwhelmingly agree ...which might include training or teaching others, research, and policy development. Every worker at every...
  18. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly...such as: Sander JD & Joung JK, Nature Biotech, 2 March 2014. Or browse the articles related to the most...
  19. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...you choose? Addgene Senior Scientist Marcy Patrick says researchers can ask themselves a few questions ...
  20. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...reverse-complement using existing software (a quick internet search will lead you to here and many others). If we put...Completed Plasmids Find the Perfect Plasmid for Your Research ...
  21. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo...Combining in silico and in vitro approaches enables researchers to develop safer and more effective mAb therapeutics...
  22. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...development or stress. With ribosome profiling, researchers have been able to study the effects of various...of a single mRNA over time. Both are helping researchers explore the complexity of translational control...
  23. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...Plasmid Serotype Name 99039  AAVrg pAAV-CamKII-ArchT-GFP (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1... viral protocols and videos Find AAVs for your research ...
  24. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...thanks to our guest blogger Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community...
  25. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...really drives creativity and novelty in scientific research and in any workspace. Re-design lab meetings with...aside from doing science, he has assisted many researchers in gaining the confidence to launch new and diverse...
  26. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...tasks to lab mates or to discuss a potential new research direction you’d like to pursue? Undefined scope... Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts Resources...
  27. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her management style... a Molecular Biologist? Find Plasmids for Your Research Here!        ...
  28. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian... Marcy Patrick...
  29. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...better interpretation. Priyamvada Prathima has a research background in protein mutagenesis and cancer immunotherapies... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School...
  30. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...
  31. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... interested in using Cpf1 multiplexing in your research? Check out the Cpf1 multiplexing plasmids available...Multiplexing Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide...
  32. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... of a biosensor Some of the biosensors used in research are very sophisticated, but at their core they...example, calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists...
  33. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...features with gamma-retrovirus including genome architecture, host integration, and its association with ...reviewed the common viruses used in biological research, you might have an idea which ones could be helpful...
  34. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...expression, or poor scalability.  Scientists at Janelia Research Campus and the University of California, Berkeley...AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...
  35. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... cell. Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough...are four main types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated...
  36. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...most rigorous, insightful, and novel scientific research will be wasted if you cannot convince others that...grant, this means convincing a committee that your research proposal is the perfect fit to their institutional...
  37. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...Coming from a strong background of undergraduate research straight to a lab career at a biotech company,...final report. Work on a final report for a user research project I led. It’s divided into the key takeaway...
  38. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...the many cells that don’t. One way to begin the search is by using selection strategies, where only cells...can hydrolyse lactose. Fortunately, for the lab researcher, when the substrate X-gal is broken down by 𝛽-galactosidase...
  39. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... ON state, and how you might use these in your research. When you're done with this post, check out our...steroid regulated promoters commonly used in plant research. Promoter Subtype Promoter Example Activator...
  40. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV genome design...Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of...
Showing: 501 - 550 of 796 results